Overview

  • Product name

    Anti-MAGEA10 antibody
  • Description

    Rabbit polyclonal to MAGEA10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 320-369 (GSDPRSFPLW YEEALKDEEE RAQDRIATTD DTTAMASASS SATGSFSYPE) of human MAGEA10 (NP_001011543).

  • Positive control

    • Fetal heart lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by peptide affinity chromatography method.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance

    This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. MAGEA10 contains one MAGE domain. Its function is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. MAGEA10 is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • Database links

  • Alternative names

    • Cancer/testis antigen 1.10 antibody
    • cancer/testis antigen family 1, member 10 antibody
    • CT1.10 antibody
    • MAGE 10 antigen antibody
    • MAGE10 antibody
    • Melanoma antigen family A 10 antibody
    • Melanoma associated antigen 10 antibody
    see all

Images

  • Anti-MAGEA10 antibody (ab83557) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 41 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Gel concentration 12%

References

ab83557 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83557.
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