Anti-MAGEA2 antibody - Aminoterminal end (ab71786)
Key features and details
- Rabbit polyclonal to MAGEA2 - Aminoterminal end
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAGEA2 antibody - Aminoterminal end
See all MAGEA2 primary antibodies -
Description
Rabbit polyclonal to MAGEA2 - Aminoterminal end -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A KLH conjugated synthetic peptide selected from the N terminal region of human MAGEA2.
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Positive control
- Human breast carcinoma tissue and HL60 lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IHC-P |
Notes |
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IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 35 kDa plus many other bands (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation. -
Tissue specificity
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. -
Sequence similarities
Contains 1 MAGE domain. -
Cellular localization
Nucleus. Nucleus, PML body. - Information by UniProt
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Database links
- Entrez Gene: 266740 Human
- Entrez Gene: 4101 Human
- Omim: 300173 Human
- Omim: 300549 Human
- SwissProt: P43356 Human
- Unigene: 534597 Human
- Unigene: 713061 Human
- Unigene: 736992 Human
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Alternative names
- Cancer/testis antigen 1.2 antibody
- Cancer/testis antigen family 1, member 2 antibody
- CT1.2 antibody
see all
Images
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Anti-MAGEA2 antibody - Aminoterminal end (ab71786) at 1/100 dilution + HL60 lysate at 12.5 µg
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with ab71786 at a 1/50 dilution. ab71786 was peroxidase conjugated to the secondary antibody, followed by AEC staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab71786 has not yet been referenced specifically in any publications.