Key features and details
- Rabbit polyclonal to MAGEA2 - Aminoterminal end
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAGEA2 antibody - Aminoterminal end
See all MAGEA2 primary antibodies
DescriptionRabbit polyclonal to MAGEA2 - Aminoterminal end
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
A KLH conjugated synthetic peptide selected from the N terminal region of human MAGEA2.
- Human breast carcinoma tissue and HL60 lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab71786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 35 kDa plus many other bands (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMAGEA2 is an antigen recognized on melanoma by autologous cytolytic T lymphocytes. The function of MAGEA2 is not known, though it may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes.
- Cancer/testis antigen 1.2 antibody
- Cancer/testis antigen family 1, member 2 antibody
- CT1.2 antibody
Anti-MAGEA2 antibody - Aminoterminal end (ab71786) at 1/100 dilution + HL60 lysate at 12.5 µg
Predicted band size: 35 kDa
Observed band size: 35 kDa
Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with ab71786 at a 1/50 dilution. ab71786 was peroxidase conjugated to the secondary antibody, followed by AEC staining.
ab71786 has not yet been referenced specifically in any publications.