Key features and details
- Rabbit polyclonal to MAGEA4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAGEA4 antibody
See all MAGEA4 primary antibodies
DescriptionRabbit polyclonal to MAGEA4
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human MAGEA4 (internal sequence). The exact sequence is proprietary.
Database link: P43358
- WB: A549 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: A-375 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionNot known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Sequence similaritiesContains 1 MAGE domain.
- Information by UniProt
- Cancer/testis antigen 1.4 antibody
- CT1.4 antibody
- MAGA4_HUMAN antibody
Paraffin-embedded human breast cancer tissue stained for MAGEA4 using ab229011 at 1/500 dilution in immunohistochemical analysis.
Anti-THEM2 antibody (ab228011) at 1/2000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 35 kDa
12% SDS-PAGE gel.
4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for MAGEA4 (green) using ab229011 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229011 has not yet been referenced specifically in any publications.