Overview

  • Product name

  • Description

    Rabbit polyclonal to MAGEA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human MAGEA4 (internal sequence). The exact sequence is proprietary.
    Database link: P43358

  • Positive control

    • WB: A549 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: A-375 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • Sequence similarities

    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 1.4 antibody
    • CT1.4 antibody
    • MAGA4_HUMAN antibody
    • MAGE 4 antibody
    • MAGE 4 antigen antibody
    • MAGE 41 antibody
    • MAGE 41 antigen antibody
    • MAGE 4A antibody
    • MAGE 4B antibody
    • MAGE X2 antibody
    • MAGE X2 antigen antibody
    • MAGE-4 antigen antibody
    • MAGE-41 antigen antibody
    • MAGE-X2 antigen antibody
    • MAGE4 antibody
    • MAGE41 antibody
    • MAGE4A antibody
    • MAGE4B antibody
    • MAGEA 4 antibody
    • MAGEA4 antibody
    • Melanoma antigen family A 4 antibody
    • Melanoma associated antigen 4 antibody
    • Melanoma-associated antigen 4 antibody
    • MGC21336 antibody
    see all

Images

  • Paraffin-embedded human breast cancer tissue stained for MAGEA4 using ab229011 at 1/500 dilution in immunohistochemical analysis.

  • Anti-THEM2 antibody (ab228011) at 1/2000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 35 kDa



    12% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for MAGEA4 (green) using ab229011 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229011 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229011.
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