Key features and details
- Mouse monoclonal [OTI1F9] to MAGEA4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-MAGEA4 antibody [OTI1F9]
See all MAGEA4 primary antibodies
DescriptionMouse monoclonal [OTI1F9] to MAGEA4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human MAGEA4 aa 1-317. Produced in HEK-293T cells (NP_001011550).
MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGT LEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSP DAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPV IFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKT GLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQD WVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRI AYPSLREAALLEEEEGV
Database link: P43358
- IHC-P: Human bladder carcinoma tissue. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY MAGEA4 cDNA.
Clone OTI1F9 (formerly 1F9).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab139297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/4000. Predicted molecular weight: 35 kDa.|
FunctionNot known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Sequence similaritiesContains 1 MAGE domain.
- Information by UniProt
- Cancer/testis antigen 1.4 antibody
- CT1.4 antibody
- MAGA4_HUMAN antibody
All lanes : Anti-MAGEA4 antibody [OTI1F9] (ab139297) at 1/4000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY MAGEA4 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 35 kDa
Paraffin-embedded human bladder carcinoma tissue stained for MAGEA4 using ab139297 at 1/150 dilution in immunohistochemical analysis.
ab139297 has been referenced in 1 publication.
- Matoso A et al. Spectrum of findings in orchiectomy specimens of persons undergoing gender confirmation surgery. Hum Pathol 76:91-99 (2018). IHC ; Human . PubMed: 29555572