Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16258] to MAGEA9
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Human
Product nameAnti-MAGEA9 antibody [EPR16258]
See all MAGEA9 primary antibodies
DescriptionRabbit monoclonal [EPR16258] to MAGEA9
Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human MAGEA9 aa 1-150. The exact sequence is proprietary.
Database link: P43362
- K562 cell lysate: K562 cells; Human testis and melanoma lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 35 kDa.|
FunctionNot known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Sequence similaritiesContains 1 MAGE domain.
- Information by UniProt
- Cancer/testis antigen 1.9 antibody
- Cancer/testis antigen family 1 member 9 antibody
- CT1.9 antibody
Immunoprecipitation analysis of human testis tissue lysate labeling MAGEA9 using ab191568 at 1/100 dilution (Left Lane). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Right Lane: PBS instead of human testis tissue lysate.
Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution + K562 cell lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
Flow cytometric analysis of 562 cells labeling MAGEA9 using ab191568 at 1/350 dilution (red) or rabbit monoclonal IgG as isotype control (green). Secondary antibody Goat anti rabbit IgG (FITC). Cells were fixed in 2% paraformaldehyde.
All lanes : Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human melanoma lysate
Lysates/proteins at 10 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 35 kDa
5% NFDM/TBST blocking buffer
ab191568 has not yet been referenced specifically in any publications.