Overview

  • Product name

    Anti-MAGEA9 antibody [EPR16258]
    See all MAGEA9 primary antibodies
  • Description

    Rabbit monoclonal [EPR16258] to MAGEA9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MAGEA9 aa 1-150. The exact sequence is proprietary.
    Database link: P43362

  • Positive control

    • K562 cell lysate: K562 cells; Human testis and melanoma lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16258
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/350.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/100.
WB 1/1000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
  • Sequence similarities

    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 1.9 antibody
    • Cancer/testis antigen family 1 member 9 antibody
    • CT1.9 antibody
    • MAGA9_HUMAN antibody
    • MAGE 9 antigen antibody
    • MAGE-9 antigen antibody
    • MAGE9 antibody
    • MAGEA9A antibody
    • MAGEA9B antibody
    • Melanoma antigen family A 9 antibody
    • Melanoma associated antigen 9 antibody
    • Melanoma-associated antigen 9 antibody
    • MGC8421 antibody
    see all

Images

  • Immunoprecipitation analysis of human testis tissue lysate labeling MAGEA9 using ab191568 at 1/100 dilution (Left Lane). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Right Lane: PBS instead of human testis tissue lysate.

  • Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution + K562 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

  • Flow cytometric analysis of 562 cells labeling MAGEA9 using ab191568 at 1/350 dilution (red) or rabbit  monoclonal IgG as isotype control (green). Secondary antibody Goat anti rabbit IgG (FITC). Cells were fixed in 2% paraformaldehyde.

  • All lanes : Anti-MAGEA9 antibody [EPR16258] (ab191568) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human melanoma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa



    5% NFDM/TBST blocking buffer

References

ab191568 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191568.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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