Recombinant
RabMAb

Recombinant Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free (ab223794)

Overview

  • Product name

    Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free
    See all MAGEC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19064] to MAGEC2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UBF1

  • Positive control

    • WB: A-375 whole cell lysate; Full length recombinant protein MAGEE1. IHC-P: Human testis, gastric cancer and melanoma tissues. ICC/IF: A-375 cells. Flow Cyt: A-375 cells. IP: A-375 whole cell lysate.
  • General notes

    Ab223794 is the carrier-free version of ab209667. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab223794 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19064
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 41 kDa).

Target

  • Tissue specificity

    Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression.
  • Sequence similarities

    Contains 1 MAGE domain.
  • Developmental stage

    Strongly expressed in spermatogonia and primary spermatocytes. At later stages of maturation, expression gradually decreases and becomes undetectable in mature spermatids.
  • Cellular localization

    Cytoplasm. Nucleus. Nuclear in germ cells. Cytoplasmic in well-differentiated hepatocellular carcinoma, nuclear in moderately- and poorly-differentiated hepatocellular carcinoma.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer testis antigen 10 antibody
    • Cancer/testis antigen 10 antibody
    • CT10 antibody
    • HCA587 antibody
    • Hepatocellular cancer antigen 587 antibody
    • Hepatocellular carcinoma associated antigen 587 antibody
    • Hepatocellular carcinoma-associated antigen 587 antibody
    • MAGC2_HUMAN antibody
    • MAGE C2 antigen antibody
    • MAGE E1 antigen antibody
    • MAGE family member C2 antibody
    • MAGE-C2 antigen antibody
    • MAGE-E1 antigen antibody
    • MAGEC2 antibody
    • Melanoma antigen family C 2 antibody
    • Melanoma antigen family C2 antibody
    • Melanoma antigen family E 1 cancer/testis specific antibody
    • Melanoma associated antigen C2 antibody
    • Melanoma-associated antigen C2 antibody
    • MGC13377 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human germ cells is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human stomach. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on melanoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MAGEC2 with ab209667 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear and weakly cytoplasmic staining on A-375 cell line

    Negative expression in HeLa cells.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).


    The negative controls are as follows:
    -ve control 1: ab209667 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling MAGEC2 with ab209667 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

  • MAGEC2 was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab209667 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209667 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: A-375 whole cell lysate 10µg (Input).

    Lane 2: ab209667 IP in A-375 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209667 in A-375 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209667).

  • This IHC data was generated using the same anti-MAGEC2 antibody clone [EPR19064] in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (cat# ab209667).

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Chen X  et al. Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer. Oncol Lett 13:1609-1618 (2017). IHC-P ; Human . Read more (PubMed: 28454298) »
See 1 Publication for this product

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