Key features and details
- Rabbit polyclonal to MAGEE2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAGEE2 antibody
See all MAGEE2 primary antibodies
DescriptionRabbit polyclonal to MAGEE2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Human colon tissue; MAGEE2 over-expression HEK293T lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Sequence similaritiesContains 2 MAGE domains.
- Information by UniProt
- HCA3 antibody
- Hepatocellular carcinoma-associated protein 3 antibody
- Hepatocellular carcinoma-associated protein HCA32 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122118 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-MAGEE2 antibody (ab122118) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique.
ab122118, at 1/50 dilution, staining MAGEE2 in paraffin embedded Human colon tissue by Immunohistochemistry.
ab122118 has not yet been referenced specifically in any publications.