Key features and details
- Rabbit polyclonal to MAGI3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAGI3 antibody
See all MAGI3 primary antibodies
DescriptionRabbit polyclonal to MAGI3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment, corresponding to amino acids 716-979 of Human MAGI3 (BC130409).
- Human fetal brain, fetal kidney and fetal liver lysates; Human fetal testis tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in 200 µl sterile distilled water.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab118615 is purified by protein affinity column.
Our Abpromise guarantee covers the use of ab118615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 130, 160 kDa (predicted molecular weight: 123, 160 kDa).|
|IHC-P||1/100 - 1/500.|
FunctionActs as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 6 PDZ (DHR) domains.
Contains 2 WW domains.
modificationsUbiquitinated following interaction with HPV E6 protein, leading to its degradation by the proteasome. Degradation is independent of E6AP ubiquitin ligase complex.
Cellular localizationCell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions.
- Information by UniProt
- dJ730K3.2 antibody
- MAGI-3 antibody
- magi3 antibody
All lanes : Anti-MAGI3 antibody (ab118615) at 1/500 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal liver lysate
Predicted band size: 123, 160 kDa
Observed band size: 130,160 kDa why is the actual band size different from the predicted?
ab118615 at 1/100 dilution, staining MAGI3 in formalin fixed, paraffin embedded Human fetal testis tissue by Immunohistochemistry. Note membrane and nuclear staining.
ab118615 has not yet been referenced specifically in any publications.