Overview

  • Product name

    Magnesium Assay Kit
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Quantitative
  • Sensitivity

    40 µM
  • Range

    2 µM - 15 µM
  • Assay time

    0h 30m
  • Product overview

    Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg2+ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 µM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Magnesium Assay Buffer WM 1 x 25ml
    Magnesium Developer Purple 1 unit
    Magnesium Enzyme Mix Green 1 unit
    Magnesium Standard Yellow 1 x 0.1ml
  • Research areas

  • Relevance

    Magnesium is the 11th most abundant element by mass in the human body. Magnesium is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Magnesium is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Magnesium compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
  • Alternative names

    • Mg++
    • Mg+2
    • Mg2+

Images

  • Magnesium measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Magnesium measured in biological fluids showing quantity (nmol) per microliter of tested sample

  • Colourimetric standard curve : mean of duplicates (+/-SD) with background reads substracted

  • Magnesium standard curve: Assay is performed according to kit protocol. Vertical dotted lines indicate the lower and upper limits of normal serum Magnesium concentrations.

Protocols

References

This product has been referenced in:

  • Zhang LW & Warrington JP Magnesium Sulfate Prevents Placental Ischemia-Induced Increases in Brain Water Content and Cerebrospinal Fluid Cytokines in Pregnant Rats. Front Neurosci 10:561 (2016). Rat . Read more (PubMed: 28008305) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Question
Answer

Thank you for getting in touch. I am happy to provide answers to your questions:

1. According to the website the linear range of detection for this kit is between 0.1-25 mM? What about higher that?

It is possible to detect higher amount as well- however, we have not tested it and it may be above the accurate range of the assay.

2. Will other ions interfere with the measurements? Meaning presence of other divalent or monovalent ions?

ab102506 Magnesium Detection Kit did not give interference from other ions tested- Zn, Mn, Ni, Fe, Cu, Co, Ca

ab102505 Calcium Detection Kit (Colorimetric) not known to show interference from Fe+2 or Mg

3. In my solution there are also presence of MOPS ions and NaCl.  

In testing, for both kits ab102505 and ab102506 we didn’t see an issue with NaCl , however, MOPS was not tested.

4. What is the binding molecule that binds to the Ca ions? In Sigma it is the o-cresolphthalein molecule. Is the binding of your molecule overcome other interaction with other ions? Meaning that the binding is specific and the presence of other ions do not interfere with the measurement.

For the calcium assay ab102505 we have a proprietary buffer formulation that prevents interference from Fe and Mg.

5. I'm also looking to measure the concertation of Mg and Zn. I found Magnesium Assay Kit, does it works with different binding molecule? Do they interfere with one another. Is it specific as well?

6. What is the binding molecule in the Mg kit?

There are no binding molecules in the magnesium assay kit ab102506  - the assay is based on a reaction where Mg serves as a co-factor.


7. Is it possible to buy the binding molecule and not the entire kit?


Unfortunately, I am sorry this is not possible in this case.


I am happy to answer any further questions you have.

Read More

Answer

I am happy to hear that the kit is working for you! Please do not hesitate to contact me if I can be of any further assistance.

Read More

Answer

Thank you for your reply.


We have rechecked the QC and retested the kit which is working as expected, therefore it is unclear why the kit is not working well in your hands. As this kit is covered by the Abpromise I am happy to offer a replacement or credit. Please let me know which you would prefer.

I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions or concerns.

Read More

Answer

Thank you for your reply.


The lab has some additional questions: How was theenzyme reconstituted/stored? Can you also describe how standards were prepared?


We want to be sure we cover all possibilities since the QC looks great for this lot. I look forward to your reply so that I may assist you further.

Read More

Answer

Thank you for your patience as I have investigated this problem further.


Based on the data you show it looks like the issue is thatno reaction mix or old/expired mix was used as there is not even the hint of a difference between 0 and the rest of the standards.


How old is this kit? When was it first reconstituted? Storage temp after? Have you tried to repeat the assay fresh (not the replicates shown)?


Also, can you please provide a lot number and order number for this kit?


I look forward to your reply so that I may assist you further.

Read More

Question
Answer

I am sorry to hear that this kit is not providing satisfactory results.

Could you please send me the data in an Excel format so we may take a look and make any suggestions as necessary? The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up