Recombinant Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Magnetic beads Llama monoclonal to GFP VHH Single Domain
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Magnetic beads
Overview
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Product name
Magnetic beads Anti-GFP VHH Single Domain antibody
See all GFP VHH Single Domain primary antibodies -
Description
Magnetic beads Llama monoclonal to GFP VHH Single Domain -
Host species
Llama -
Conjugation
Magnetic beads -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein. ab193983 is a VHH single domain antibody targeting GFP
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Positive control
- IP: HEK293 whole cell lysate - Overexpressing GFP, Recombinant GFP protein and GFP Fusion protein.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 2.92% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab193983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. |
Target
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Relevance
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Absorption: Abs(max)=395 nm. Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. -
Alternative names
- GFP antibody
- Green fluorescent protein antibody
- yfp antibody
Images
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Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)
Lane 1: Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul recombinant GFP (ab84191)
Lane 2: Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul GFP fusion protein.
Lane 3: Off-target sdAb beads: Anti-Rabbit VHH Single Domain Antibody (Magnetic) + 10ul recombinant GFP (ab84191)
Lane 4: Anti-GFP antibody (ab290) + Protein A agarose + 10ul recombinant GFP (ab84191)
Lane 5: Anti-GFP antibody (ab290) + Protein A agarose + 10ul fusion GFP
Anti-GFP antibody (Magnetic beads) (ab193983) (20ul slurry) was used to pull down recombinant GFP (ab84191) and GFP fusion protein. ab290 (Rabbit polyclonal to GFP - ChIP Grade) and Protein A agarose was used as a positive control. The gel was stained with Optiblot Blue (ab119211).
Lanes 1-2 : Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983) at 20 µl (Bead Slurry)
Lanes 4-5 : Anti-GFP antibody (ab290) at 1/500 dilution
All lanes :
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/20000 dilution
Observed band size: 27 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)Lane 1: HEK293 cell lysate overexpressing GFP - 10ug
Lane 2: Unbound IP supernatant: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 3: Negative IP: off-target sdAb beads: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 4: IP: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 5: HEK293 cell lysate overexpressing GFP - 10ug
Lane 6: Unbound IP supernatant: anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP
Lane 7: IP: anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP
Anti-GFP antibody (Magnetic beads) (ab193983) was used to pull down GFP from HEK293 cell lysate overexpressing GFP. ab290 (Rabbit polyclonal to GFP - ChIP Grade) was used as a positive control. The gel was transferred to Nitrocellulose membrane and probed with mouse anti-GFP (ab1218) and anti-mouse HRP (ab97040).
All lanes : Anti-GFP antibody [9F9.F9] (ab1218) at 1/500 dilution
All lanes : HEK293 whole cell lysate - Overexpressing GFP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/20000 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)Lane 1: HEK293 cell lysate overexpressing GFP - 10ug
Lane 2: Unbound IP supernatant: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 3: Negative IP: off-target sdAb beads: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 4: IP: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 5: HEK293 cell lysate overexpressing GFP - 10ug
Lane 6: Unbound IP supernatant: anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP
Lane 7: IP: anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP
Anti-GFP antibody (Magnetic beads) (ab193983) was used to pull down GFP from HEK293 cell lysate overexpressing GFP. ab290 (Rabbit polyclonal to GFP - ChIP Grade) was used as a positive control. The gel was stained with Optiblot Blue (ab119211).
All lanes : Anti-GFP antibody [9F9.F9] (ab1218) at 1/500 dilution
All lanes :
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/20000 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted? -
Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)
References (1)
ab193983 has been referenced in 1 publication.
- Lee S et al. Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis. Neuron 91:41-55 (2016). IP . PubMed: 27321923
