Recombinant Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Magnetic beads Llama monoclonal to GFP VHH Single Domain
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Magnetic beads
Overview
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Product name
Magnetic beads Anti-GFP VHH Single Domain antibody -
Description
Magnetic beads Llama monoclonal to GFP VHH Single Domain -
Host species
Llama -
Conjugation
Magnetic beads -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein. ab193983 is a VHH single domain antibody targeting GFP
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Positive control
- IP: HEK293 whole cell lysate - Overexpressing GFP, Recombinant GFP protein and GFP Fusion protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 2.92% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
Target
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Relevance
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Absorption: Abs(max)=395 nm. Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. -
Alternative names
- GFP antibody
- Green fluorescent protein antibody
- yfp antibody
Images
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Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)
Lane 1: Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul recombinant GFP (ab84191)
Lane 2: Anti-GFP antibody (Magnetic beads) (ab193983) + 10ul GFP fusion protein.
Lane 3: Off-target sdAb beads: Anti-Rabbit VHH Single Domain Antibody (Magnetic) + 10ul recombinant GFP (ab84191)
Lane 4: Anti-GFP antibody (ab290) + Protein A agarose + 10ul recombinant GFP (ab84191)
Lane 5: Anti-GFP antibody (ab290) + Protein A agarose + 10ul fusion GFP
Anti-GFP antibody (Magnetic beads) (ab193983) (20ul slurry) was used to pull down recombinant GFP (ab84191) and GFP fusion protein. ab290 (Rabbit polyclonal to GFP - ChIP Grade) and Protein A agarose was used as a positive control. The gel was stained with Optiblot Blue (ab119211).
Lanes 1-2 : Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983) at 20 µl (Bead Slurry)
Lanes 4-5 : Anti-GFP antibody (ab290) at 1/500 dilution
All lanes :
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/20000 dilution
Observed band size: 27 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)Lane 1: HEK293 cell lysate overexpressing GFP - 10ug
Lane 2: Unbound IP supernatant: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 3: Negative IP: off-target sdAb beads: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 4: IP: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 5: HEK293 cell lysate overexpressing GFP - 10ug
Lane 6: Unbound IP supernatant: anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP
Lane 7: IP: anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP
Anti-GFP antibody (Magnetic beads) (ab193983) was used to pull down GFP from HEK293 cell lysate overexpressing GFP. ab290 (Rabbit polyclonal to GFP - ChIP Grade) was used as a positive control. The gel was transferred to Nitrocellulose membrane and probed with mouse anti-GFP (ab1218) and anti-mouse HRP (ab97040).
All lanes : Anti-GFP antibody [9F9.F9] (ab1218) at 1/500 dilution
All lanes : HEK293 whole cell lysate - Overexpressing GFP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/20000 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)Lane 1: HEK293 cell lysate overexpressing GFP - 10ug
Lane 2: Unbound IP supernatant: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 3: Negative IP: off-target sdAb beads: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 4: IP: Anti-GFP antibody (Magnetic beads) + 100ug HEK293 cell lysate overexpressing GFP
Lane 5: HEK293 cell lysate overexpressing GFP - 10ug
Lane 6: Unbound IP supernatant: anti-GFP antibody (ab290) + Protein A agarose + 100ug HEK293 cell lysate overexpressing GFP
Lane 7: IP: anti-GFP antibody (ab290) + Protein A agarose+ 100ug HEK293 cell lysate overexpressing GFP
Anti-GFP antibody (Magnetic beads) (ab193983) was used to pull down GFP from HEK293 cell lysate overexpressing GFP. ab290 (Rabbit polyclonal to GFP - ChIP Grade) was used as a positive control. The gel was stained with Optiblot Blue (ab119211).
All lanes : Anti-GFP antibody [9F9.F9] (ab1218) at 1/500 dilution
All lanes :
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/20000 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted? -
Magnetic beads Anti-GFP VHH Single Domain antibody (ab193983)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab193983 has been referenced in 1 publication.
- Lee S et al. Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis. Neuron 91:41-55 (2016). IP . PubMed: 27321923