Product nameMagnetic Purification System - AbPure™
Magnetic Purification System - AbPure™ (ab270532) is a convenient method to purify antibodies at a small scale with high recoveries. It quickly removes substances such as BSA, glycine, tris and azide that interfere with conjugation reactions. It can be easily adapted to your needs allowing you to purify from 20 to 200 μg of antibody and to reach the desired final antibody concentration. The antibody to be purified or cleaned up should ideally be in a volume of 20 μl to 1 ml.
No dialysis of the antibody is needed prior to conjugation as interfering substances such as Tris, citrate and glycine have been replaced with conjugation-friendly alternatives.
This sytem can directly replace any existing protein A purification kit and can be used to isolate IgG antibodies from crude mixtures.
- Less than 15 minutes hands on time.
- 70-90% antibody recovery.
- Up to 10 purifications at the smallest working scale.
This product is manufactured by Expedeon, an Abcam company. It was previously called AbPure™ Universal Magnetic Purification System. Expedeon product code 265-0200 is the same as the 1 kit size of this product.
Antibody amount (µg) Beads volume (µl) Elution volume (µl) Neutralization volume (µl) up to 20 40 20 2 up to 50 100 50 5 up to 100 200 100 10 up to 200 400 200 20
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 kit AbPure™ Mag 10X Binding Buffer 1 vial AbPure™ Mag Elution Buffer 1 vial AbPure™ Mag Neutralizaton Buffer 1 vial AbPure™ Mag Wash Buffer 1 unit Protein A Magnetic Beads 1 x 400µl
The method involves capturing the antibody on the Protein A Magnetic Beads. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species; once the antibody has bound to the Protein A, unwanted substances can be removed by simply collecting the beads in a magnetic stand and washing the beads. The antibody is then eluted and neutralized.
A sample of rabbit serum has been purified by using Magnetic Purification System -AbPure™ (ab270532).
The gel picture shows how all the contaminants are effectively removed and the purified rabbit IgG is eluted.
20 µg (A), 25 µg (B) or 50 µg (C) rabbit IgG was purified using Magnetic Purification System - AbPure™ (ab270532), demonstrating high recoveries in the first elution fraction even at the smallest working scale.
ab270532 has not yet been referenced specifically in any publications.