Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14037] to MAGOH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-MAGOH antibody [EPR14037]
See all MAGOH primary antibodies
DescriptionRabbit monoclonal [EPR14037] to MAGOH
Specificityab180505 detects both Mago nashi homolog and Mago nashi homolog 2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human MAGOH aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P61326
- Hela, Raji, Jurkat, HL60 cell lysates, Human endometrial adenocarcinoma tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionComponent of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.
Sequence similaritiesBelongs to the mago nashi family.
Cellular localizationNucleus. Nucleus speckle. Cytoplasm. Detected in granule-like structures in the dendroplasm (By similarity). Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
- Information by UniProt
- Mago nashi homolog proliferation associated (Drosophila) antibody
- Mago nashi protein homolog antibody
- magoh antibody
All lanes : Anti-MAGOH antibody [EPR14037] (ab180505) at 1/2000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HL60 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Blocking buffer: 5% NFDM/TBST
Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling Mago nashi homolog 2 with ab180505 at 1/250 dilution. Secondary ab: HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab180505 has been referenced in 1 publication.
- Blazquez L et al. Exon Junction Complex Shapes the Transcriptome by Repressing Recursive Splicing. Mol Cell 72:496-509.e9 (2018). PubMed: 30388411