Overview

  • Product name

    Anti-MAGOH antibody [EPR14037]
    See all MAGOH primary antibodies
  • Description

    Rabbit monoclonal [EPR14037] to MAGOH
  • Host species

    Rabbit
  • Specificity

    ab180505 detects both Mago nashi homolog and Mago nashi homolog 2
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human MAGOH aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P61326

  • Positive control

    • Hela, Raji, Jurkat, HL60 cell lysates, Human endometrial adenocarcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the mago nashi family.
  • Cellular localization

    Nucleus. Nucleus speckle. Cytoplasm. Detected in granule-like structures in the dendroplasm (By similarity). Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • Mago nashi homolog proliferation associated (Drosophila) antibody
    • Mago nashi protein homolog antibody
    • magoh antibody
    • MAGOHA antibody
    • MGN_HUMAN antibody
    • Protein mago nashi homolog antibody
    see all

Images

  • All lanes : Anti-MAGOH antibody [EPR14037] (ab180505) at 1/2000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HL60 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Blocking buffer: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling Mago nashi homolog 2 with ab180505 at 1/250 dilution. Secondary ab: HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Blazquez L  et al. Exon Junction Complex Shapes the Transcriptome by Repressing Recursive Splicing. Mol Cell 72:496-509.e9 (2018). Read more (PubMed: 30388411) »
See 1 Publication for this product

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