Anti-MAGP1 antibody (ab231344)
Key features and details
- Rabbit polyclonal to MAGP1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-MAGP1 antibody
See all MAGP1 primary antibodies -
Description
Rabbit polyclonal to MAGP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Cow -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human MAGP1 aa 1 to the C-terminus. (Expressed in E.coli)
Database link: P55001 -
Positive control
- IHC-P: Human breast cancer, brain, prostate, prostate cancer and colorectal cancer tissues. WB: Recombinant human MAGP1 protein; Pig liver lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
Target
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Function
Component of the elastin-associated microfibrils. -
Sequence similarities
Belongs to the MFAP family.
Contains 1 SXC (ShKT) domain. -
Post-translational
modificationsForms intermolecular disulfide bonds either with other MAGP-1 molecules or with other components of the microfibrils. May form transglutaminase cross-links.
O-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 281912 Cow
- Entrez Gene: 4237 Human
- Omim: 156790 Human
- SwissProt: P27424 Cow
- SwissProt: P55001 Human
- Unigene: 389137 Human
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Alternative names
- MAGP antibody
- MAGP-1 antibody
- MAGP1 antibody
see all
Images
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Anti-MAGP1 antibody (ab231344) at 2 µg/ml + Pig liver tissue lysate.
Developed using the ECL technique.
Predicted band size: 21 kDa -
Anti-MAGP1 antibody (ab231344) at 2 mg/ml + Recombinant human MAGP1 protein.
Developed using the ECL technique.
Predicted band size: 21 kDa -
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for MAGP1 using ab231344 at 20 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for MAGP1 using ab231344 at 20 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for MAGP1 using ab231344 at 20 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human brain tissue stained for MAGP1 using ab231344 at 20 µg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for MAGP1 using ab231344 at 20 µg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab231344 has been referenced in 2 publications.
- Xie Y et al. COPS3 Promotes Proliferation, Invasion, and EMT of Colorectal Cancer Cells by MEK/ERK Pathway. Dis Markers 2022:7594489 (2022). PubMed: 35903293
- Qin C et al. Novel Molecular Hallmarks of Group 3 Medulloblastoma by Single-Cell Transcriptomics. Front Oncol 11:622430 (2021). PubMed: 33816256