Recombinant Anti-MAGP2 antibody [EPCSUR1] (ab171737)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPCSUR1] to MAGP2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MAGP2 antibody [EPCSUR1]
See all MAGP2 primary antibodies -
Description
Rabbit monoclonal [EPCSUR1] to MAGP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human MAGP2. The exact sequence is proprietary.
Database link: Q13361 -
Positive control
- MAGP2 transfected 293T cell lysates; Human colon and kidney tissues.
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General notes
This antibody was developed as part of a collaboration between the National Institutes of Health and the lab of Alison Miyomoto at California State University, Fullerton.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPCSUR1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 20 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the elastin-associated microfibrils. -
Sequence similarities
Belongs to the MFAP family. -
Post-translational
modificationsForms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 8076 Human
- Omim: 601103 Human
- SwissProt: Q13361 Human
- Unigene: 512842 Human
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Alternative names
- AAT9 antibody
- MAGP 2 antibody
- MAGP-2 antibody
see all
Images
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Immunohistochemistry of paraffin-embedded Human kidney tissue labeling MAGP2 using ab171737 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded Human colon tissue labeling MAGP2 using ab171737 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-MAGP2 antibody [EPCSUR1] (ab171737) at 1/1000 dilution
Lane 1 : Non transfected 293T cell lysate
Lane 2 : MAGP2 transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab171737 has been referenced in 1 publication.
- Feifei W et al. MAGP2, a Component of Extracellular Matrix, Is Upregulated in Colorectal Cancer and Negatively Modulated by miR-200b-3p. Technol Cancer Res Treat 18:1533033819870777 (2019). PubMed: 31426719