Overview

  • Product name

    Anti-MAGP2 antibody [EPR17581]
    See all MAGP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR17581] to MAGP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human MAGP2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13361

  • Positive control

    • WB: Human bladder lysate; HeLa cell lysate; Rat kidney and spleen lysates. IHC-P: Human colon and breast carcinoma tissues. ICC/IF: HeLa and A673 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 20 kDa).
IP 1/200.
Flow Cyt 1/750.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Component of the elastin-associated microfibrils.
    • Sequence similarities

      Belongs to the MFAP family.
    • Post-translational
      modifications

      Forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • AAT9 antibody
      • MAGP 2 antibody
      • MAGP-2 antibody
      • MAGP2 antibody
      • MFAP 5 antibody
      • MFAP-5 antibody
      • MFAP5 antibody
      • MFAP5_HUMAN antibody
      • Microfibril associated glycoprotein 2 antibody
      • Microfibril-associated glycoprotein 2 antibody
      • microfibrillar associated protein 5 antibody
      • Microfibrillar associated protein 5 precursor antibody
      • Microfibrillar-associated protein 5 antibody
      • MP25 antibody
      see all

    Images

    • Anti-MAGP2 antibody [EPR17581] (ab203828) at 1/10000 dilution + Human bladder lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 20 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-MAGP2 antibody [EPR17581] (ab203828) at 1/5000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 20 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-MAGP2 antibody [EPR17581] (ab203828) at 1/1000 dilution

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 20 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAGP2 with ab203828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human colon tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MAGP2 with ab203828 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    • Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MAGP2 with ab203828 at 1/750 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    • MAGP2 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab203828 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab203828 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

      Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab203828 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203828 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

    References

    ab203828 has not yet been referenced specifically in any publications.

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