Key features and details
- Rabbit polyclonal to MAK16
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAK16 antibody
DescriptionRabbit polyclonal to MAK16
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
- IHC-P: Human cerebellum tissue WB: Transfected HEK-293T whole cell lysate; ICC: A-431 whole cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Cellular localizationNuclear; nucleolus
- MAK16 homolog (S. cerevisiae) antibody
- MAK16 homolog antibody
- MAK16L antibody
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling MAK16 in the nucleolus of Purkinje cells using ab185101 at a 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-MAK16 antibody (ab185101) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MAK16 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 35 kDa
Immunocytochemistry analysis of A431 (human epidermoid carcinoma cell line) whole cells labeling MAK16 in the nucleolus of Purkinje cells using ab185101 at 2 µg/ml.
ab185101 has not yet been referenced specifically in any publications.