Key features and details
- Rabbit polyclonal to MAL
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Dog, Human, Frog
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20861303|
|IP||Use at an assay dependent concentration. PubMed: 20861303|
FunctionCould be an important component in vesicular trafficking cycling between the Golgi complex and the apical plasma membrane. Could be involved in myelin biogenesis and/or myelin function.
Sequence similaritiesBelongs to the MAL family.
Contains 1 MARVEL domain.
Developmental stageExpressed in the intermediate and late stages of T-cell differentiation.
- Information by UniProt
- mal antibody
- MAL protein gene antibody
- Mal T-cell differentiation protein antibody
All lanes : Anti-MAL antibody (ab15418) at 1/5000 dilution
Lane 1 : mpkCCD cell lysate
Lane 2 : Xenopus 2f3 cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : HRP conjugated goat anti-rabbit
Predicted band size: 18 kDa
IHC image of ab15418 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab15418, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab15418 has been referenced in 2 publications.
- Carmosino M et al. MAL/VIP17, a new player in the regulation of NKCC2 in the kidney. Mol Biol Cell 21:3985-97 (2010). ICC/IF, IP ; Rat, Dog . PubMed: 20861303
- Kamsteeg EJ et al. MAL decreases the internalization of the aquaporin-2 water channel. Proc Natl Acad Sci U S A 104:16696-701 (2007). IP ; Rat . PubMed: 17940053