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    malaria-elisa-kit-ab178649.pdf

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Microbiology Organism Eukaryotic Microorganism Protozoa Plasmodium
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Anti-Malaria ELISA Kit (ab178649)

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  • SDS
  • Protocol Booklet
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Serologic ELISA assay principle

    Key features and details

    • Sensitivity: 95.9 %
    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

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    Overview

    • Product name

      Anti-Malaria ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Positive 22 2.8%
      Positive 22 3.9%
      Negative 22 5.5%
      Inter-assay
      Sample n Mean SD CV%
      Positive 24 3.2%
      Positive 24 4.8%
      Negative 24 10.3%
    • Sample type

      Serum, Hep Plasma, Cit plasma
    • Assay type

      Indirect
    • Sensitivity

      = 95.9 %
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s anti-Malaria Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG and IgM class antibodies against Malaria in Human serum and plasma.


      A 96-well plate has been precoated with Malaria antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG and anti-Human IgM conjugate is added to the wells, which binds to the immobilized Malaria antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Malaria IgG or Malaria IgM sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      20X Washing Solution White cap 1 x 50ml
      Cover Foil 1 unit
      IgG Sample Diluent 1 x 100ml
      Malaria Anti-IgG and Anti-IgM HRP Conjugate 1 x 20ml
      Malaria Coated Microplate (12 x 8 wells) 1 unit
      Malaria Cut-off Control 1 x 3ml
      Malaria Negative Control 1 x 2ml
      Malaria Positive Control 1 x 2ml
      Stop Solution red cap 1 x 15ml
      Strip holder 1 unit
      TMB Substrate Solution Yellow cap 1 x 15ml
    • Research areas

      • Microbiology
      • Organism
      • Eukaryotic Microorganism
      • Protozoa
      • Plasmodium
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Pathogen specific immunoglobulins ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Infectious disease ELISA kits

    Associated products

      Images

      • Serologic ELISA assay principle
        Serologic ELISA assay principle
        Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab178649? Please let us know so that we can cite the reference in this datasheet.

      ab178649 has not yet been referenced specifically in any publications.

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