Anti-Malaria ELISA Kit (ab178649)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Malaria ELISA Kit
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Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Positive 22 2.8% Positive 22 3.9% Negative 22 5.5% Inter-assay Sample n Mean SD CV% Positive 24 3.2% Positive 24 4.8% Negative 24 10.3% -
Sample typeSerum, Heparin Plasma, Citrate Plasma
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Assay typeIndirect
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Sensitivity= 95.9 %
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam’s anti-Malaria Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG and IgM class antibodies against Malaria in Human serum and plasma.
A 96-well plate has been precoated with Malaria antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG and anti-Human IgM conjugate is added to the wells, which binds to the immobilized Malaria antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Malaria IgG or Malaria IgM sample captured in plate.
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Tested applicationsSuitable for: Indirect ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Malaria Anti-IgG and Anti-IgM HRP Conjugate 1 x 20ml Malaria Coated Microplate (12 x 8 wells) 1 unit Malaria Cut-off Control 1 x 3ml Malaria Negative Control 1 x 2ml Malaria Positive Control 1 x 2ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Applications
Our Abpromise guarantee covers the use of ab178649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA | Use at an assay dependent concentration. |
Protocols
References
ab178649 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"