Product nameMaleimide-Alkaline Phosphatase
Maleimide-Alkaline Phosphatase (ab270022) is designed to facilitate the conjugation of Alkaline Phosphatase to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to Alkaline Phosphatase allows conjugation to be performed very efficiently at physiological pH.
This product is manufactured by Expedeon, an Abcam company. It was previously called Maleimide-Alkaline Phosphatase. Expedeon product code 402-0002 is the same as the 2 mg size of this product.
The buffer in which the thiolated molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed.
Please see the below table for recommended buffer conditions and components:
Buffer components Compatible pH range 6.5-7.5 Yes Chelating agents (e.g. EDTA) No Primary amines Yes Reducing reagents No Sodium Azide <0.1%
Molecules that do not contain –SH groups can be chemically modified to introduce the required thiol functionality.
Abcam offers a thiolation kit (ab270020) compatible with this product.
In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. Any reducing reagent (e. g. dithiothreitol and β-mercaptoethanol) used to produce free thiols should be removed before proceeding with the conjugation.
- Allow the vial of maleimide-activated Alkaline Phosphatase to warm to room temperature.
- Remove the crimp seal and reconstitute the lyophilized powder by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
- Incubate for 3-4 hours (or overnight) at room temperature and then store at 4°C.
Storage instructionsShipped at room temperature. Store at -20°C.
Storage bufferConstituent: 10% ALKALINE PHOSPHATASE
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270022 has not yet been referenced specifically in any publications.