Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Suspension cells
- Sensitivity: 10 pg/well
Product nameMaleimide Quantification Assay Kit (Colorimetric)
See all Maleimide kits
Sample typeCell culture extracts, Adherent cells, Suspension cells
ab112140 assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M -1cm-1. The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups. This spectrophotometric assay for the determination of maleimide groups is a reverse GSH assay. It takes advantage of the high reactivity of thiols of GSH with the maleimide moiety. Maleimide of the sample is allowed to form a stable thiosuccinimidyl linkage with GSH. After the reaction of the sample is complete, the excess GSH, i.e., the remaining thiols of GSH in the reaction mixture, is estimated by using 4,4'-DTDP. The amount of GSH reacted with the sample is titrated to determine the extent of maleimide. For more sensitive maleimide quantitation, we recommend that you use our fluorimetric kit ab112141 that has higher sensitivity.
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Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests 4,4’-DTDP Component B 1 vial Assay Buffer 1 x 50ml DMSO 1 x 1ml MEA Component A 1 vial
ab112140 has not yet been referenced specifically in any publications.