Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
Product nameMaleimide Quantification Assay Kit (Fluorometric)
See all Maleimide kits
ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorometric method to detect as little as 10 picomoles of maleimide in a 100 µL assay volume (100 nM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. Compared to ab112140, this fluorometric assay is more sensitive, and has less interference from biological samples.
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ab112141 should be stored desiccated.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 25ml DMSO 1 x 200µl MalemGreen indicator 1 vial N-ethylmaleimide Standard 1 x 50µl Reaction Buffer 1 x 500µl
ab112141 has been referenced in 1 publication.
- Hurst CH et al. Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods. Biotechniques 62:69-75 (2017). PubMed: 28193150