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Based on the Q&A you provide in your website regarding this kit, the pH of the reaction buffer is pretty basic. Because my polymer can't dissolve in that high pH, i am afraid that my polymer will just precipitate out once i added the reaction buffer to my polymer solution. So is any buffer i can use to replace the reaction buffer you provide in the kit?? I would like to use the buffer that is close to neutral (7 to 8) if possible...
Asked on May 03 2012
Thank you for contacting Abcam regarding ab112141.
The reaction buffer, after adding it into the assay buffer has a pH of ˜7-7.5, so it shouldn't be a problem.
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Answered on May 03 2012