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i am getting background of 10000 fluoresence units staying within the 30 min incubation, is this too high?
for data analysis, am I supposed to be taking the log of both the x and y axis to plot the data points?
Asked on Jan 16 2013
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The readings are really depending on the instruments. We use Molecular Devices's Gemini fluorescent plate reader, the background reading is around 1000-2000, the reading at 10 uM maleimide is ˜4000-7000.
For data analysis, you can use linear, Log-log or 4 parameter depending on which one give you the best fitted curve. Hope it is helpful. Please let us know if you have any questions.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jan 16 2013