Product nameMaleimide-R Phycoerythrin
Maleimide-Activated R Phycoerythrin (ab270023) is designed to facilitate the conjugation of R-Phycoerythrin to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to R-Phycoerythrin allows conjugation to be performed very efficiently at physiological pH.
This product is manufactured by Expedeon, an Abcam company. It was previously called Maleimide-R Phycoerythrin (2mg)SKU: 403-0002. Expedeon product code 403-0002 is the same as the 2 mg size of this product.
The buffer in which the thiolated molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. EDTA (1-10 mM) may help to prevent metal catalyzed oxidation of thiols.
Please see the below table for recommended buffer conditions and components:
Buffer components Compatible pH range 6.5-7.5 Yes Chelating agents (e.g. EDTA) Yes Primary amines Yes Reducing reagents No Sodium Azide <0.1%
Molecules that do not contain –SH groups can be chemically modified to introduce the required thiol functionality.
Abcam offers a thiolation kit (ab270020) compatible with this product.
In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. Any reducing reagent (e. g. dithiothreitol and β-mercaptoethanol) used to produce free thiols should be removed before proceeding with the conjugation.
Storage instructionsShipped at room temperature. Store at -20°C.
Storage bufferpH: 5.8
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270023 has not yet been referenced specifically in any publications.