Product nameMaleimide-R Phycoerythrin
Maleimide-R-Phycoerythrin (ab270023) is designed to facilitate the conjugation of R-Phycoerythrin to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to R-Phycoerythrin allows conjugation to be performed very efficiently at physiological pH.
This product is manufactured by Expedeon, an Abcam company. It was previously called Maleimide-R Phycoerythrin (2mg)SKU: 403-0002. Expedeon product code 403-0002 is the same as the 2 mg size of this product.
The buffer in which the thiolated molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. EDTA (1-10 mM) may help to prevent metal catalyzed oxidation of thiols.
Please see the below table for recommended buffer conditions and components:
Buffer components Compatible pH range 6.5-7.5 Yes Chelating agents (e.g. EDTA) Yes Primary amines Yes Reducing reagents No Sodium Azide <0.1%
Molecules that do not contain –SH groups can be chemically modified to introduce the required thiol functionality.
Abcam offers a thiolation kit (ab270020) compatible with this product.
In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. Any reducing reagent (e. g. dithiothreitol and β-mercaptoethanol) used to produce free thiols should be removed before proceeding with the conjugation.
- Allow the vial of maleimide-activated R-Phycoerythrin to warm to room temperature in the dark.
- Remove the crimp seal and reconstitute the lyophilized powder by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
- Incubate for 3-4 hours (or overnight) at room temperature in the dark and then store at 4°C in the dark.
Storage instructionsShipped at room temperature. Store at -20°C.
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ab270023 has not yet been referenced specifically in any publications.