Overview

  • Product name
    Anti-Malondialdehyde antibody
    See all Malondialdehyde primary antibodies
  • Description
    Rabbit polyclonal to Malondialdehyde
  • Host species
    Rabbit
  • Specificity
    This antibody specifically binds to Malondialdehyde LDL and other Malondialdehyde modified proteins.
  • Tested applications
    Suitable for: ELISA, WB, Conjugationmore details
  • Immunogen

    Chemical/ Small Molecule MDA modified protein

Properties

Applications

Our Abpromise guarantee covers the use of ab27642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/40000.
WB 1/5000 - 1/40000.
Conjugation Use at an assay dependent dilution.

Target

  • Relevance
    Malondialdehyde (MDA) is a natural product formed in all mammalian cells as a product of lipid peroxidation. MDA is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. It reacts with DNA to form adducts to deoxyguanosine and deoxyadenosine. The major adduct to DNA is a pyrimidopurinone called M1G which appears to be a major endogenous DNA adduct in human beings that may contribute significantly to cancer linked to lifestyle and dietary factors. MDA modified proteins may show altered physico chemical behavior and antigenicity. MDA is toxic and has been implicated in aging mutagenesis, carcinogenesis, diabetic nephropathy and radiation damage. Increased expression of MDA has been reported in the brains of Alzheimer's patients. Antibodies to MDA will help to visualize the MDA adducts.
  • Alternative names
    • 1133 Tetramethoxypropane antibody
    • MDA antibody

Images

  • All lanes : Anti-Malondialdehyde antibody (ab27642) at 1/1000 dilution (in PBST for 18 hours at 4°C)

    Lane 1 : Whole tissue lysate of raw cashew nuts
    Lane 2 : Whole tissue lysate of roasted cashew nuts

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : An HRP-conjugated goat anti-rabbit polyclonal

    Developed using the ECL technique.

    Observed band size: 10-12 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    Blocking Step: 2% Milk for 30 minutes at 25°C

References

This product has been referenced in:
  • Tiao MM  et al. Resveratrol ameliorates maternal and post-weaning high-fat diet-induced nonalcoholic fatty liver disease via renin-angiotensin system. Lipids Health Dis 17:178 (2018). Read more (PubMed: 30055626) »
  • Carnuta MG  et al. Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients. Sci Rep 7:7295 (2017). Read more (PubMed: 28779156) »
See all 12 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your inquiry. As this is a small molecule, there is no species reactivity associated with it. We expect the antibody to recognize Malondialdehyde regardless of the source. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse penile tissue)
Loading amount
60 µg
Specification
Mouse penile tissue
Gel Running Conditions
Reduced Denaturing (4-20% mini gel)
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Miss. Gwen Lagoda

Verified customer

Submitted Feb 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Human Penile Tissue)
Loading amount
60 µg
Specification
Human Penile Tissue
Gel Running Conditions
Reduced Denaturing (4-20% mini gel)
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Miss. Gwen Lagoda

Verified customer

Submitted Jan 30 2012

Answer

Thank you for contacting us.

I would suggest analyzing the entire lane. The MDA-modifications and their extent of it will vary from sample to sample as well as from species to species.

It might very well be that in cashews there is only one major protein that has this modification. But in samples from other species the MDA modificationscan be present for a wider range of proteins.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cashew nuts (Anacardiaceae) Tissue lysate - whole (whole nut)
Loading amount
50 µg
Specification
whole nut
Treatment
roasted
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C

Mr. Chris Mattison

Verified customer

Submitted Oct 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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