• Product name

    Anti-MALT1/MLT antibody [EP603Y]
    See all MALT1/MLT primary antibodies
  • Description

    Rabbit monoclonal [EP603Y] to MALT1/MLT
  • Host species

  • Specificity

    This antibody is predicted to detect splice isoform 2 based on sequence analysis.
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MALT1/MLT aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Ramos, HeLa and Jurkat whole cell lysate (ab7899). ICC/IF: Ramos cells. Flow Cyt: Jurkat cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


     This product was previously labelled as MALT1


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab33921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
Flow Cyt 1/100.

For unpurified use at 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion.
    • Tissue specificity

      Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung.
    • Involvement in disease

      Note=A chromosomal aberration involving MALT1 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with BIRC2. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
    • Sequence similarities

      Belongs to the peptidase C14B family.
      Contains 1 death domain.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Cytoplasm > perinuclear region. Nucleus. Shuttles between the nucleus and cytoplasm. Found in perinuclear structures together with BCL10.
    • Information by UniProt
    • Database links

    • Alternative names

      • Caspase like protein antibody
      • DKFZp434L132 antibody
      • IMD12 antibody
      • MALT 1 antibody
      • MALT associated translocation antibody
      • MALT lymphoma associated translocation antibody
      • MALT lymphoma-associated translocation antibody
      • Malt1 antibody
      • MALT1 paracaspase antibody
      • MALT1_HUMAN antibody
      • MLT 1 antibody
      • MLT antibody
      • MLT1 antibody
      • Mucosa associated lymphoid tissue lymphoma translocation gene 1 antibody
      • Mucosa associated lymphoid tissue lymphoma translocation protein 1 antibody
      • Mucosa-associated lymphoid tissue lymphoma translocation protein 1 antibody
      • Paracaspase antibody
      • Paracaspase-1 antibody
      • PCASP1 antibody
      see all


    • All lanes : Anti-MALT1/MLT antibody [EP603Y] (ab33921) at 1/10000 dilution (purified)

      Lane 1 : Ramos cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 92 kDa
      Observed band size: 92 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling MALT1/MLT with purified ab33921 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Anti-MALT1/MLT antibody [EP603Y] (ab33921) at 1/2000 dilution (unpurified) + Jurkat cell lysate

      Predicted band size: 92 kDa
      Observed band size: 92 kDa

    • Flow Cytometry analysis of Jurkat cells labelling MALT1/MLT with purified ab33921 at 1/100 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Overlay histogram showing Jurkat cells stained with unpurified ab33921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab33921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


    This product has been referenced in:

    • Ginster S  et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS One 12:e0169026 (2017). WB . Read more (PubMed: 28052131) »
    • Afonina IS  et al. The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes. EMBO Rep 17:914-27 (2016). Read more (PubMed: 27113748) »
    See all 7 Publications for this product

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