Recombinant Anti-MALT1/MLT antibody [EP603Y] (ab33921)
![Western blot - Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-389527-anti-malt1mlt-antibody-ep603y-western-blot-human-knockout-lysate.jpg "Western blot - Anti-MALT1/MLT antibody [EP603Y] (ab33921)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP603Y] to MALT1/MLT
- Suitable for: Flow Cyt (Intra), IP, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MALT1/MLT antibody [EP603Y]
See all MALT1/MLT primary antibodies -
Description
Rabbit monoclonal [EP603Y] to MALT1/MLT -
Host species
Rabbit -
Specificity
This antibody is predicted to detect splice isoform 2 based on sequence analysis. -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MALT1/MLT aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UDY8 -
Positive control
- WB: Ramos, HeLa, K562. Jurkat whole cell lysate (ab7899). ICC/IF: Ramos cells. Flow Cyt (intra): Jurkat cells. IP: Ramos whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP603Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
For unpurified use at 1/100 - 1/1000.ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP |
1/50.
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
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| ICC/IF |
1/250.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/100. For unpurified use at 1/100 - 1/1000.ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP
1/50. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 92 kDa. |
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ICC/IF
1/250. |
Target
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Function
Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. -
Tissue specificity
Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung. -
Involvement in disease
Note=A chromosomal aberration involving MALT1 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with BIRC2. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma. -
Sequence similarities
Belongs to the peptidase C14B family.
Contains 1 death domain.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cytoplasm > perinuclear region. Nucleus. Shuttles between the nucleus and cytoplasm. Found in perinuclear structures together with BCL10. - Information by UniProt
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Database links
- Entrez Gene: 10892 Human
- Omim: 604860 Human
- SwissProt: Q9UDY8 Human
- Unigene: 601217 Human
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Alternative names
- Caspase like protein antibody
- DKFZp434L132 antibody
- IMD12 antibody
see all
Images
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Western blot - Anti-MALT1/MLT antibody [EP603Y] (ab33921)All lanes : Anti-MALT1/MLT antibody [EP603Y] (ab33921) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MALT1 knockout HeLa cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDaLanes 1-3: Merged signal (red and green). Green - ab33921 observed at 92 kDa. Red - loading control ab7291 observed at 50 kDa.
ab33921 Anti-MALT1/MLT antibody [EP603Y] was shown to specifically react with MALT1/MLT in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264930 (knockout cell lysate ab257149) was used. Wild-type and MALT1/MLT knockout samples were subjected to SDS-PAGE. ab33921 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-255376-anti-malt1-antibody-ep603y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MALT1/MLT antibody [EP603Y] (ab33921)Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling MALT1/MLT with purified ab33921 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: Primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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![Immunoprecipitation - Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-477462-AntiMALT1MLTantibodyEP603Yimmunoprecipitationramoshuman.jpg)
Immunoprecipitation - Anti-MALT1/MLT antibody [EP603Y] (ab33921)MALT1/MLT was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab33921 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab33921 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Ramos whole cell lysate 10 µg (Input).
Lane 2: ab33921 IP in Ramos whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab33921 in Ramos whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-250246-anti-malt1-antibody-ep603y-western-blot.jpg)
Western blot - Anti-MALT1/MLT antibody [EP603Y] (ab33921)All lanes : Anti-MALT1/MLT antibody [EP603Y] (ab33921) at 1/10000 dilution (purified)
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-250275-anti-malt1-antibody-ep603y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-MALT1/MLT antibody [EP603Y] (ab33921)Intracellular Flow Cytometry analysis ofJurkat (Human T cell leukemia cell line from peripheral blood) cells labelling MALT1/MLT with purified ab33921 at 1/100 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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![Western blot - Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-47314-anti-malt1-antibody-ep603y-western-blot.jpg)
Western blot - Anti-MALT1/MLT antibody [EP603Y] (ab33921)Anti-MALT1/MLT antibody [EP603Y] (ab33921) at 1/2000 dilution (unpurified) + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 92 kDa
Observed band size: 92 kDa -
![Flow Cytometry (Intracellular) - Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-210408-anti-malt1-antibody-ep603y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-MALT1/MLT antibody [EP603Y] (ab33921)Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with unpurified ab33921 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab33921, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Anti-MALT1/MLT antibody [EP603Y] (ab33921)](https://www.abcam.com/ps/products/33/ab33921/Images/ab33921-9-benefits-of-recombinant-antibodies.png)
Anti-MALT1/MLT antibody [EP603Y] (ab33921)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab33921 has been referenced in 9 publications.
- Tsui KH et al. Mucosa-Associated Lymphoid Tissue 1 Is an Oncogene Inducing Cell Proliferation, Invasion, and Tumor Growth via the Upregulation of NF-?B Activity in Human Prostate Carcinoma Cells. Biomedicines 9:N/A (2021). PubMed: 33802402
- Demeyer A et al. MALT1 Proteolytic Activity Suppresses Autoimmunity in a T Cell Intrinsic Manner. Front Immunol 10:1898 (2019). PubMed: 31474984
- Ginster S et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS One 12:e0169026 (2017). WB . PubMed: 28052131
- Afonina IS et al. The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes. EMBO Rep 17:914-27 (2016). PubMed: 27113748
- Yang YK et al. Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling. J Biol Chem 291:25921-25936 (2016). PubMed: 27777308
- Baens M et al. MALT1 auto-proteolysis is essential for NF-?B-dependent gene transcription in activated lymphocytes. PLoS One 9:e103774 (2014). WB . PubMed: 25105596
- Chan W et al. A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH domains that induce Bcl10 ubiquitination and human lymphoma cell survival. Mol Cell Biol 33:429-43 (2013). WB ; Human . PubMed: 23149938
- Chng WJ et al. Gene expression profiling of pulmonary mucosa-associated lymphoid tissue lymphoma identifies new biologic insights with potential diagnostic and therapeutic applications. Blood 113:635-45 (2009). PubMed: 18974375
- McCully RR & Pomerantz JL The protein kinase C-responsive inhibitory domain of CARD11 functions in NF-kappaB activation to regulate the association of multiple signaling cofactors that differentially depend on Bcl10 and MALT1 for association. Mol Cell Biol 28:5668-86 (2008). PubMed: 18625728