Recombinant
RabMAb

Recombinant Anti-MALT1/MLT antibody [EP603Y] (Phycoerythrin) (ab210982)

Overview

  • Product name

    Anti-MALT1/MLT antibody [EP603Y] (Phycoerythrin)
    See all MALT1/MLT primary antibodies
  • Description

    Rabbit monoclonal [EP603Y] to MALT1/MLT (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MALT1/MLT aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9UDY8

  • Positive control

    • Flow Cyt: HeLa cells ICC/IF: HeLa cells
  • General notes

     This product was previously labelled as MALT1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab210982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/50. This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Function

    Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion.
  • Tissue specificity

    Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung.
  • Involvement in disease

    Note=A chromosomal aberration involving MALT1 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with BIRC2. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
  • Sequence similarities

    Belongs to the peptidase C14B family.
    Contains 1 death domain.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Cytoplasm > perinuclear region. Nucleus. Shuttles between the nucleus and cytoplasm. Found in perinuclear structures together with BCL10.
  • Information by UniProt
  • Database links

  • Alternative names

    • Caspase like protein antibody
    • DKFZp434L132 antibody
    • IMD12 antibody
    • MALT 1 antibody
    • MALT associated translocation antibody
    • MALT lymphoma associated translocation antibody
    • MALT lymphoma-associated translocation antibody
    • Malt1 antibody
    • MALT1 paracaspase antibody
    • MALT1_HUMAN antibody
    • MLT 1 antibody
    • MLT antibody
    • MLT1 antibody
    • Mucosa associated lymphoid tissue lymphoma translocation gene 1 antibody
    • Mucosa associated lymphoid tissue lymphoma translocation protein 1 antibody
    • Mucosa-associated lymphoid tissue lymphoma translocation protein 1 antibody
    • Paracaspase antibody
    • Paracaspase-1 antibody
    • PCASP1 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab210982 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210982, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.

  • ab210982 staining MALT1/MLT in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210982 at 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

This product has been referenced in:

See 1 Publication for this product

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