Overview

  • Product name

    Maltose and Glucose Assay Kit
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Sensitivity

    > 0.01 nmol/well
  • Range

    0.01 nmol/well - 10 nmol/well
  • Assay time

    1h 00m
  • Product overview

    Maltose and Glucose Assay Kit (ab65335) can measure glucose or maltose levels in a variety of biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). The assay is based on the ability of glucose oxidase to specifically oxidize free glucose, producing a product that interacts with the glucose probe. The amount of product can be either colorimetric (spectrophotometry at OD= 570 nm) or fluorometric (Ex/Em = 535/587 nm) method.

    This kit can detect 10 pmol to 10 nmol glucose per assay. It can also be used for isomaltose detection.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glucose (C6H12O6; FW: 180.16) and Maltose (C12H22O11; FW: 342.3) are the main fuel sources to generate the universal energy molecule ATP. Maltose is the major disaccharide that generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    alpha-D-Glucosidase (Lyophilized) Blue 1 vial
    Assay Buffer WM 1 x 25ml
    Glucose Enzyme Mix (lyophilized) Green 1 vial
    Glucose Probe Red 1 x 0.2ml
    Maltose Standard (100 nmol/µl) Yellow 1 x 100µl
  • Research areas

  • Relevance

    Maltose (C12H22O11; fW:342.30) is a disaccharide formed from two units of glucose (C6H12O6; FW: 180.16) joined via a a-1,4-glycosidic linkage. Maltose is the major disaccharide generated from hydrolysis of starch and in living organisms, maltose is hydrolyzed by maltase. Once inside the cells, glucose will be processed and ener the glycolysis pathway to ultimately produce enery in form of ATP.

Images

  • Maltose Standard Curve. Assays were performed using various amounts of Maltose standard according to kit instructions. Circle is maltose with a-D-Glucosidase. Square is maltose without a-D-Glucosidase. Triangle is glucose with 2 times nmol of Maltose.

Protocols

References

ab65335 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Kit ab65335 has not yet been tested specifically with dog samples. We have not previously used these kits to measure maltase activity, however this may be possible with some protocol optimization.

We do also carry the following two kits which may be of interest:

Maltose Assay Kit (ab83388)

Glucose Assay Kit (ab65333)

Read More

Question
Answer

The alpha-D-Glucosidase included in kit ab65335 is from Saccharomyces cerevisiae.

Read More

Answer

The following products are meant for detection of total amount of sample, NOT enzyme activity. However, the kits may be able to be used in reverse - meaning that the enzyme in your sample will be used to convert a known amount of substrate (standard) and later be quantified. Please bear in mind that this still gives a read out in amount of sample detected, and is correlated to the amount of enzyme in your sample.

1) Malate Dehydrogenase: ab83391 Malate Assay Kit
In the assay, malate is specifically oxidized by using an enzyme mix to generate a product which reacts with WST substrate probe to generate color for detection.

2) Maltose Hydrolase: ab83388 Maltose Assay Kit, ab65335 Maltose and Glucose Assay Kit
The same theory applies here, where the glucose found in maltose is used to produce a product that interacts with the glucose probe to generate color and fluorescence.

3) CoA Synthetase: ab8756 PicoProbe™ Acetyl CoA Assay Kit
Acetyl CoA is converted to CoA, then reacted to form NADH which interacts with PicoProbe to generate fluorescence.

Read More

Answer

Product Maltose and Glucose Assay Kit (ab65335) may be suitable for you.   Maltose contains two glucose units joined by an alpha-1,4-glycosidic linkage, which can be easily converted to two glucoses by alpha-D-glucosidase. Glucose oxidase specifically oxidizes free glucose to produce a product that interacts with the glucose probe to generate color and fluorescence.

Therefore, glucose or maltose levels in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.) can be easily determined by either colorimetric (spectrophotometry at OD = 570 nm) or fluorometric (ex/em = 535/587 nm) methods. The assay can detect 10 pmol to 10 nmol glucose per assay.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up