Maltose Assay Kit (ab83388)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Maltose Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
Abcam's Maltose Assay Kit provides a fast, easy and sensitive method for quantifying maltose in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.).
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K628 Maltose Colorimetric/Fluorometric Assay Kit. K628-100 is the same size as the 100 test size of ab83388.
Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase. The generated glucose can be specifically oxidized to produce a product that interacts with the probe to generate color and fluorescence. Thus, maltose can be determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests a-D-Glucosidase (Lyophilized) 1 vial Enzyme Mix (Lyophilized) 1 vial Maltose Assay Buffer 1 x 25ml Maltose Probe in DMSO 1 x 200µl Maltose Standard (100 nmol/µl) 1 x 100µl -
Research areas
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Relevance
Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. -
Alternative names
- 4 O alpha D Glucopyranosyl D glucose
- Malt sugar
Images
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Maltose with α-D-Glucosidase Standard Curve. Assays were performed following kit instructions
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Maltose Standard Curve. Assays were performed following kit instructions. Open circle is maltose with alpha-D-Glucosidase. Solid square is maltose without alpha-D-Glucosidase. Triangle is free glucose (divided by 2).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab83388 has been referenced in 1 publication.
- Axelsson E et al. The genomic signature of dog domestication reveals adaptation to a starch-rich diet. Nature N/A:N/A (2013). Functional Studies . PubMed: 23354050