Overview

  • Product name

    Maltose Assay Kit
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Assay time

    1h 00m
  • Product overview

    Abcam's Maltose Assay Kit provides a fast, easy and sensitive method for quantifying maltose in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a a-1,4-glycosidic linkage, which can be easily converted to two glucoses by a-D-glucosidase. The generated glucose can be specifically oxidized to produce a product that interacts with the probe to generate color and fluorescence. Thus, maltose can be determined by either colorimetric (spectrophotometry at ? = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    a-D-Glucosidase (Lyophilized) Blue 1 vial
    Enzyme Mix (Lyophilized) Green 1 vial
    Maltose Assay Buffer WM 1 x 25ml
    Maltose Probe in DMSO Red 1 x 200µl
    Maltose Standard (100 nmol/µl) Yellow 1 x 100µl
  • Research areas

  • Relevance

    Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food.
  • Alternative names

    • 4 O alpha D Glucopyranosyl D glucose
    • Malt sugar

Images

  • Maltose with α-D-Glucosidase Standard Curve. Assays were performed following kit instructions

  • Maltose Standard Curve. Assays were performed following kit instructions. Open circle is maltose with alpha-D-Glucosidase. Solid square is maltose without alpha-D-Glucosidase. Triangle is free glucose (divided by 2).

Protocols

References

This product has been referenced in:

  • Axelsson E  et al. The genomic signature of dog domestication reveals adaptation to a starch-rich diet. Nature N/A:N/A (2013). Functional Studies . Read more (PubMed: 23354050) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Extraction of proteins is not necessary, as kit ab83388 measures maltose. Samples in ethanol are not compatible as the enzymes in the kit work in aqueous solution.

Tissue and cell samples can be homogenized in 2-3 volumes of the Maltose Assay Buffer, and then centrifuged for 5-10 minutes to remove debris; the supernatant is the lysate. Several volumes of this lysate can then be tested to choose a volume or dilution that yields results within the linear range of the standard curve.

Read More

Answer

1) Malate Assay Kit (ab83391): detects product formed.
Step 1: Malate in sample + oxidation = product formed.
Step 2: Product formed + probe = color generation.

2) Maltose Assay Kit (ab83388): detects product formed.
Step 1: Maltose in sample + alpha D glucosidase = two glucose units.
Step 2: Two glucose units + oxidation = product formed. Step 3: Product formed + probe = color generation.

3) Amylase Assay Kit (ab102523): direct detection/amount in sample.
Step 1: Amylase in sample + substrate = fluorescence.

4) Pyruvate Assay Kit (ab65342): detects product formed.
Step 1: Pyruvatein sample + oxidation = product formed.
Step 2: Product formed + probe = color generation.

Read More

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