Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Product nameMaltose Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay time1h 00m
Abcam's Maltose Assay Kit provides a fast, easy and sensitive method for quantifying maltose in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.).
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Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase. The generated glucose can be specifically oxidized to produce a product that interacts with the probe to generate color and fluorescence. Thus, maltose can be determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests a-D-Glucosidase (Lyophilized) Blue 1 vial Enzyme Mix (Lyophilized) Green 1 vial Maltose Assay Buffer WM 1 x 25ml Maltose Probe in DMSO Red 1 x 200µl Maltose Standard (100 nmol/µl) Yellow 1 x 100µl
RelevanceMaltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food.
- 4 O alpha D Glucopyranosyl D glucose
- Malt sugar
Maltose with α-D-Glucosidase Standard Curve. Assays were performed following kit instructions
Maltose Standard Curve. Assays were performed following kit instructions. Open circle is maltose with alpha-D-Glucosidase. Solid square is maltose without alpha-D-Glucosidase. Triangle is free glucose (divided by 2).
ab83388 has been referenced in 1 publication.
- Axelsson E et al. The genomic signature of dog domestication reveals adaptation to a starch-rich diet. Nature N/A:N/A (2013). Functional Studies . PubMed: 23354050