Western blot abreview for Anti-Maltose Binding Protein antibody

Excellent
Abreviews
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST + 5% Milk

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit antibody (Sigma A0545)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/16000

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 47.5 kDa

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Submitted Nov 03 2009

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