Anti-Maltose Binding Protein antibody [R29.6] (ab65)
Key features and details
- Mouse monoclonal [R29.6] to Maltose Binding Protein
- Suitable for: IP, WB, ChIP
- Reacts with: Escherichia coli
- Isotype: IgG1
Overview
-
Product name
Anti-Maltose Binding Protein antibody [R29.6]
See all Maltose Binding Protein primary antibodies -
Description
Mouse monoclonal [R29.6] to Maltose Binding Protein -
Host species
Mouse -
Tested applications
Suitable for: IP, WB, ChIPmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Fusion protein corresponding to Maltose Binding Protein. MOS maltose binding protein fusion protein
-
Positive control
- MBP fusion protein generated with the pmal plasmid (New England Biolabs) in bacterial lysate.
-
General notes
MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
R29.6 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/10000.
|
ChIP | (1) |
Use at an assay dependent concentration.
|
Notes |
---|
IP
Use at an assay dependent concentration. |
WB
1/10000. |
ChIP
Use at an assay dependent concentration. |
Target
-
Relevance
Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein. -
Cellular localization
Periplasm -
Database links
- Entrez Gene: 1039564 Escherichia coli
- Entrez Gene: 4494002 Escherichia coli
- Entrez Gene: 948538 Escherichia coli
- Entrez Gene: 960089 Escherichia coli
- SwissProt: P0AEX9 Escherichia coli
- SwissProt: P0AEY0 Escherichia coli
-
Alternative names
- ECK4026 antibody
- JW3994 antibody
- Mal E antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab65 has been referenced in 8 publications.
- Li Y et al. ABNORMAL SHOOT 6 interacts with KATANIN 1 and SHADE AVOIDANCE 4 to promote cortical microtubule severing and ordering in Arabidopsis. J Integr Plant Biol 63:646-661 (2021). PubMed: 32761943
- Morrison MS et al. Disulfide-compatible phage-assisted continuous evolution in the periplasmic space. Nat Commun 12:5959 (2021). PubMed: 34645844
- Kamennaya NA & Post AF Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. Appl Environ Microbiol 77:291-301 (2011). PubMed: 21057026
- Im YJ et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). PubMed: 19686684
- Verhoeven GS et al. Differential bacterial surface display of peptides by the transmembrane domain of OmpA. PLoS One 4:e6739 (2009). WB . PubMed: 19707582
- Liu X et al. Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection. Genome Res 16:1517-28 (2006). PubMed: 17053089
- Liu X et al. DIP-chip: rapid and accurate determination of DNA-binding specificity. Genome Res 15:421-7 (2005). ChIP ; Saccharomyces cerevisiae . PubMed: 15710749
- Leliveld SR et al. Apoptin induces tumor-specific apoptosis as a globular multimer. J Biol Chem 278:9042-51 (2003). ICC/IF . PubMed: 12496278