Overview

  • Product name
    Anti-Maltose Binding Protein antibody [R29.6]
    See all Maltose Binding Protein primary antibodies
  • Description
    Mouse monoclonal [R29.6] to Maltose Binding Protein
  • Host species
    Mouse
  • Tested applications
    Suitable for: IP, WB, ChIP, ICC/IFmore details
  • Immunogen

    MOS maltose binding protein fusion protein.

  • General notes
    MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    R29.6
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 12496278

Target

  • Relevance
    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • Cellular localization
    Periplasm
  • Alternative names
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Images

  • Immunofluorescence analysis of Saos-2 cells, transfected with full-length MBP-Apoptin and MBP-Apoptin(80–121). After fixation, the cells were stained for Maltose Binding Protein using ab65. Nuclear morphology is indicated by DAPI staining.
  • Representative western blot detecting Maltose Binding Protein using ab65 at 1.8 µg/ml.

References

This product has been referenced in:
  • Kamennaya NA & Post AF Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. Appl Environ Microbiol 77:291-301 (2011). Read more (PubMed: 21057026) »
  • Im YJ  et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). Read more (PubMed: 19686684) »
See all 6 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Saccharomyces cerevisiae Cell (S. cerevisae)
Specification
S. cerevisae
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Other
Positive control
negative control: MBP-expressing vector in yeast

Mr. Xiao Liu

Verified customer

Submitted Aug 11 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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