Anti-MAML1 antibody (ab17019)

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Western blot - Anti-MAML1 antibody (ab17019)

    Key features and details

    • Goat polyclonal to MAML1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-MAML1 antibody
      See all MAML1 primary antibodies

    • Description

      Goat polyclonal to MAML1

    • Host species

      Goat

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide:

      VLPTCPMAEFALPR

      , corresponding to N terminal amino acids 2-15 of Human MAML1(Peptide available as ab23302.)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab17019 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Application Abreviews Notes
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa.

    1 hour primary incubation is recommended for this product.
    Notes
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa.

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.

    • Tissue specificity

      Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.

    • Sequence similarities

      Belongs to the mastermind family.

    • Domain

      The C-terminal region is required for transcriptional activation.

    • Cellular localization

      Nucleus speckle. Nuclear, in a punctate manner.
    • Information by UniProt

    • Database links

      • Alternative names

        • Mam-1 antibody
        • Mam1 antibody
        • MAML 1 antibody
        • Maml1 antibody
        • MAML1_HUMAN antibody
        • Mastermind like 1 antibody
        • Mastermind-like protein 1 antibody
        see all

      Images

      • Western blot - Anti-MAML1 antibody (ab17019)
        Western blot - Anti-MAML1 antibody (ab17019)
        Anti-MAML1 antibody (ab17019) at 0.1 µg/ml + Tissue lysates prepared from Human skeletal muscles at 35 µg

        Predicted band size: 108 kDa

      References (1)

      Publishing research using ab17019? Please let us know so that we can cite the reference in this datasheet.

      ab17019 has been referenced in 1 publication.

      • Wu X  et al. Crosstalk between BMP and Notch Induces Sox2 in Cerebral Endothelial Cells. Cells 8:N/A (2019). PubMed: 31174355

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