Anti-MAML1 antibody (ab17019)
Key features and details
- Goat polyclonal to MAML1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAML1 antibody
See all MAML1 primary antibodies -
Description
Goat polyclonal to MAML1 -
Host species
Goat -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide:
VLPTCPMAEFALPR
, corresponding to N terminal amino acids 2-15 of Human MAML1(Peptide available as ab23302.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
|
All applications |
Mouse
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Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. -
Tissue specificity
Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. -
Sequence similarities
Belongs to the mastermind family. -
Domain
The C-terminal region is required for transcriptional activation. -
Cellular localization
Nucleus speckle. Nuclear, in a punctate manner. - Information by UniProt
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Database links
- Entrez Gene: 9794 Human
- Entrez Gene: 103806 Mouse
- Omim: 605424 Human
- SwissProt: Q92585 Human
- SwissProt: Q6T264 Mouse
- Unigene: 631951 Human
- Unigene: 51116 Mouse
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Alternative names
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab17019 has been referenced in 1 publication.
- Wu X et al. Crosstalk between BMP and Notch Induces Sox2 in Cerebral Endothelial Cells. Cells 8:N/A (2019). PubMed: 31174355