Overview

  • Product name
    Anti-MAML1 antibody
    See all MAML1 primary antibodies
  • Description
    Goat polyclonal to MAML1
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    VLPTCPMAEFALPR

    , corresponding to N terminal amino acids 2-15 of Human MAML1(Peptide available as ab23302.)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Peptide Antibody detection limit dilution 1/128000.
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa.Can be blocked with Human MAML1 peptide (ab23302).
IHC-P Use a concentration of 2.5 - 3.8 mg/ml.

Target

  • Function
    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
  • Tissue specificity
    Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
  • Sequence similarities
    Belongs to the mastermind family.
  • Domain
    The C-terminal region is required for transcriptional activation.
  • Cellular localization
    Nucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • Database links
    • Alternative names
      • Mam-1 antibody
      • Mam1 antibody
      • MAML 1 antibody
      • Maml1 antibody
      • MAML1_HUMAN antibody
      • Mastermind like 1 antibody
      • Mastermind-like protein 1 antibody
      see all

    Images

    • Western blot - Anti-MAML1 antibody (ab17019)
      Western blot - Anti-MAML1 antibody (ab17019)
      Anti-MAML1 antibody (ab17019) at 0.1 µg/ml + Tissue lysates prepared from Human skeletal muscles at 35 µg

      Predicted band size: 108 kDa

    • Immunohistochemistry (Paraffin-embedded sections) - Anti-MAML1 antibody (ab17019)
      Immunohistochemistry (Paraffin-embedded sections) - Anti-MAML1 antibody (ab17019)
      ab17019 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    References

    ab17019 has not yet been referenced specifically in any publications.

    Publishing research using ab17019? Please let us know so that we can cite the reference in this datasheet.

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