Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab17019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Peptide Antibody detection limit dilution 1/128000.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa.Can be blocked with Human MAML1 peptide (ab23302).|
|IHC-P||Use a concentration of 2.5 - 3.8 mg/ml.|
FunctionActs as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Tissue specificityWidely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
Sequence similaritiesBelongs to the mastermind family.
DomainThe C-terminal region is required for transcriptional activation.
Cellular localizationNucleus speckle. Nuclear, in a punctate manner.
- Information by UniProt
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
ab17019 has not yet been referenced specifically in any publications.