Anti-MAML1 antibody (ab17019)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-MAML1 antibody
See all MAML1 primary antibodies -
Description
Goat polyclonal to MAML1 -
Host species
Goat -
Tested applications
Suitable for: ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide:
VLPTCPMAEFALPR
, corresponding to N terminal amino acids 2-15 of Human MAML1(Peptide available as ab23302.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab17019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. Peptide Antibody detection limit dilution 1/128000. |
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| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa. | |
| IHC-P | Use a concentration of 2.5 - 3.8 mg/ml. |
Target
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Function
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. -
Tissue specificity
Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. -
Sequence similarities
Belongs to the mastermind family. -
Domain
The C-terminal region is required for transcriptional activation. -
Cellular localization
Nucleus speckle. Nuclear, in a punctate manner. - Information by UniProt
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Database links
- Entrez Gene: 9794 Human
- Entrez Gene: 103806 Mouse
- Omim: 605424 Human
- SwissProt: Q92585 Human
- SwissProt: Q6T264 Mouse
- Unigene: 631951 Human
- Unigene: 51116 Mouse
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Alternative names
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
see all
Images
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Western blot - Anti-MAML1 antibody (ab17019)Anti-MAML1 antibody (ab17019) at 0.1 µg/ml + Tissue lysates prepared from Human skeletal muscles at 35 µg
Predicted band size: 108 kDa -
Immunohistochemistry paraffin embedded sections - Anti-MAML1 antibody (ab17019)ab17019 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Datasheets and documents
References
This product has been referenced in:
- Wu X et al. Crosstalk between BMP and Notch Induces Sox2 in Cerebral Endothelial Cells. Cells 8:N/A (2019). Read more (PubMed: 31174355) »
