Overview

  • Product name
    Anti-MAML1 antibody
    See all MAML1 primary antibodies
  • Description
    Rabbit polyclonal to MAML1
  • Host species
    Rabbit
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1

  • Positive control
    • Whole cell lysate from HeLa cells and 293T cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
    • Tissue specificity
      Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
    • Sequence similarities
      Belongs to the mastermind family.
    • Domain
      The C-terminal region is required for transcriptional activation.
    • Cellular localization
      Nucleus speckle. Nuclear, in a punctate manner.
    • Information by UniProt
    • Database links
      • Alternative names
        • Mam-1 antibody
        • Mam1 antibody
        • MAML 1 antibody
        • Maml1 antibody
        • MAML1_HUMAN antibody
        • Mastermind like 1 antibody
        • Mastermind-like protein 1 antibody
        see all

      Images

      • Western blot - Anti-MAML1 antibody (ab70486)
        Western blot - Anti-MAML1 antibody (ab70486)
        All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml

        Lane 1 : Whole cell lysate from HeLa cells at 50 µg
        Lane 2 : Whole cell lysate from HeLa cells at 15 µg
        Lane 3 : Whole cell lysate from HeLa cells at 5 µg
        Lane 4 : Whole cell lysate from 293T cells at 50 µg

        Developed using the ECL technique.

        Predicted band size: 108 kDa


        Exposure time: 30 seconds
      • Immunoprecipitation - Anti-MAML1 antibody (ab70486)
        Immunoprecipitation - Anti-MAML1 antibody (ab70486)
        Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
        Chemiluminescence with exposure times of 30 seconds.

      References

      ab70486 has not yet been referenced specifically in any publications.

      Publishing research using ab70486? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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