Anti-MAML1 antibody (ab70486)
Key features and details
- Rabbit polyclonal to MAML1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAML1 antibody
See all MAML1 primary antibodies -
Description
Rabbit polyclonal to MAML1 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested Applications & Species
Application Species IP HumanWB Human -
Immunogen
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1
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Positive control
- Whole cell lysate from HeLa cells and 293T cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
IP |
Human
|
WB |
Human
|
All applications |
Mouse
Guinea pig
Pig
Chimpanzee
Rhesus monkey
Elephant
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Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
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|
IP |
Use at 1-4 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 108 kDa. |
IP
Use at 1-4 µg/mg of lysate. |
Target
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Function
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. -
Tissue specificity
Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. -
Sequence similarities
Belongs to the mastermind family. -
Domain
The C-terminal region is required for transcriptional activation. -
Cellular localization
Nucleus speckle. Nuclear, in a punctate manner. - Information by UniProt
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Database links
- Entrez Gene: 9794 Human
- Entrez Gene: 103806 Mouse
- Omim: 605424 Human
- SwissProt: Q92585 Human
- SwissProt: Q6T264 Mouse
- Unigene: 631951 Human
- Unigene: 51116 Mouse
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Alternative names
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
see all
Images
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All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 30 seconds -
Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
Chemiluminescence with exposure times of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab70486 has not yet been referenced specifically in any publications.