The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 1-4µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
Belongs to the mastermind family.
The C-terminal region is required for transcriptional activation.
All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
Chemiluminescence with exposure times of 30 seconds.