Anti-MAML1 antibody (ab70486)

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Western blot - Anti-MAML1 antibody (ab70486)
  • Immunoprecipitation - Anti-MAML1 antibody (ab70486)

Key features and details

  • Rabbit polyclonal to MAML1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MAML1 antibody
    See all MAML1 primary antibodies

  • Description

    Rabbit polyclonal to MAML1

  • Host species

    Rabbit

  • Specificity

    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1

  • Positive control

    • Whole cell lysate from HeLa cells and 293T cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
All applications
Mouse
Guinea pig
Pig
Chimpanzee
Rhesus monkey
Elephant
Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP
Use at 1-4 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP
Use at 1-4 µg/mg of lysate.

Target

  • Function

    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.

  • Tissue specificity

    Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.

  • Sequence similarities

    Belongs to the mastermind family.

  • Domain

    The C-terminal region is required for transcriptional activation.

  • Cellular localization

    Nucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt

  • Database links

    • Alternative names

      • Mam-1 antibody
      • Mam1 antibody
      • MAML 1 antibody
      • Maml1 antibody
      • MAML1_HUMAN antibody
      • Mastermind like 1 antibody
      • Mastermind-like protein 1 antibody
      see all

    Images

    • Western blot - Anti-MAML1 antibody (ab70486)
      Western blot - Anti-MAML1 antibody (ab70486)
      All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique.

      Predicted band size: 108 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-MAML1 antibody (ab70486)
      Immunoprecipitation - Anti-MAML1 antibody (ab70486)
      Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
      Chemiluminescence with exposure times of 30 seconds.

    References (0)

    Publishing research using ab70486? Please let us know so that we can cite the reference in this datasheet.

    ab70486 has not yet been referenced specifically in any publications.

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