Product nameAnti-MAML1 antibody
See all MAML1 primary antibodies
DescriptionRabbit polyclonal to MAML1
SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Elephant
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human MAML1
- Whole cell lysate from HeLa cells and 293T cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Tissue specificityWidely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
Sequence similaritiesBelongs to the mastermind family.
DomainThe C-terminal region is required for transcriptional activation.
Cellular localizationNucleus speckle. Nuclear, in a punctate manner.
- Information by UniProt
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
All lanes : Anti-MAML1 antibody (ab70486) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 30 seconds
Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70486 at 3 µg/mg lysate for IP.
Chemiluminescence with exposure times of 30 seconds.
ab70486 has not yet been referenced specifically in any publications.