Overview

  • Product name
    Anti-MAML1 antibody - N-terminal
    See all MAML1 primary antibodies
  • Description
    Rabbit polyclonal to MAML1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-45 of Human MAML1 (Uniprot ID: Q92585).

  • Positive control
    • HeLa and Mouse heart whole cell lysates; HeLa nuclear lysate; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 89% PBS, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
  • Tissue specificity
    Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
  • Sequence similarities
    Belongs to the mastermind family.
  • Domain
    The C-terminal region is required for transcriptional activation.
  • Cellular localization
    Nucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mam-1 antibody
    • Mam1 antibody
    • MAML 1 antibody
    • Maml1 antibody
    • MAML1_HUMAN antibody
    • Mastermind like 1 antibody
    • Mastermind-like protein 1 antibody
    see all

Images

  • Anti-MAML1 antibody - N-terminal (ab155786) at 1/1000 dilution + Mouse heart whole cell lysate at 50 µg

    Predicted band size: 108 kDa



    7.5% SDS PAGE
  • All lanes : Anti-MAML1 antibody - N-terminal (ab155786) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa nuclear cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 108 kDa



    7.5% SDS PAGE
  • Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MAML1 with ab155786 at 1/500 dilution. Alpha-tubulin filaments are labeled in red.

References

ab155786 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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