Key features and details
- Rabbit polyclonal to MAML1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MAML1 antibody - N-terminal
See all MAML1 primary antibodies
DescriptionRabbit polyclonal to MAML1 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- HeLa and Mouse heart whole cell lysates; HeLa nuclear lysate; HeLa cells.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 108 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionActs as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Tissue specificityWidely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
Sequence similaritiesBelongs to the mastermind family.
DomainThe C-terminal region is required for transcriptional activation.
Cellular localizationNucleus speckle. Nuclear, in a punctate manner.
- Information by UniProt
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
Anti-MAML1 antibody - N-terminal (ab155786) at 1/1000 dilution + Mouse heart whole cell lysate at 50 µg
Predicted band size: 108 kDa
7.5% SDS PAGE
All lanes : Anti-MAML1 antibody - N-terminal (ab155786) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa
7.5% SDS PAGE
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MAML1 with ab155786 at 1/500 dilution. Alpha-tubulin filaments are labeled in red.
ab155786 has been referenced in 2 publications.
- Ferrante F et al. HDAC3 functions as a positive regulator in Notch signal transduction. Nucleic Acids Res 48:3496-3512 (2020). PubMed: 32107550
- Zheng CG et al. miR-133b Downregulation Reduces Vulnerable Plaque Formation in Mice with AS through Inhibiting Macrophage Immune Responses. Mol Ther Nucleic Acids 16:745-757 (2019). PubMed: 31146256