Anti-MAML1 antibody - N-terminal (ab155786)
Key features and details
- Rabbit polyclonal to MAML1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MAML1 antibody - N-terminal
See all MAML1 primary antibodies -
Description
Rabbit polyclonal to MAML1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide, corresponding to a region within N terminal amino acids 1-45 of Human MAML1 (Uniprot ID: Q92585).
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Positive control
- HeLa and Mouse heart whole cell lysates; HeLa nuclear lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/500 - 1/3000. Predicted molecular weight: 108 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 108 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. -
Tissue specificity
Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. -
Sequence similarities
Belongs to the mastermind family. -
Domain
The C-terminal region is required for transcriptional activation. -
Cellular localization
Nucleus speckle. Nuclear, in a punctate manner. - Information by UniProt
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Database links
- Entrez Gene: 9794 Human
- Entrez Gene: 103806 Mouse
- Omim: 605424 Human
- SwissProt: Q92585 Human
- SwissProt: Q6T264 Mouse
- Unigene: 631951 Human
- Unigene: 51116 Mouse
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Alternative names
- Mam-1 antibody
- Mam1 antibody
- MAML 1 antibody
see all
Images
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Anti-MAML1 antibody - N-terminal (ab155786) at 1/1000 dilution + Mouse heart whole cell lysate at 50 µg
Predicted band size: 108 kDa
7.5% SDS PAGE -
All lanes : Anti-MAML1 antibody - N-terminal (ab155786) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa
7.5% SDS PAGE -
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling MAML1 with ab155786 at 1/500 dilution. Alpha-tubulin filaments are labeled in red.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab155786 has been referenced in 3 publications.
- Gagliani EK et al. A Drosophila Su(H) model of Adams-Oliver Syndrome reveals cofactor titration as a mechanism underlying developmental defects. PLoS Genet 18:e1010335 (2022). PubMed: 35951645
- Ferrante F et al. HDAC3 functions as a positive regulator in Notch signal transduction. Nucleic Acids Res 48:3496-3512 (2020). PubMed: 32107550
- Zheng CG et al. miR-133b Downregulation Reduces Vulnerable Plaque Formation in Mice with AS through Inhibiting Macrophage Immune Responses. Mol Ther Nucleic Acids 16:745-757 (2019). PubMed: 31146256