Overview

  • Product name

  • Description

    Rabbit polyclonal to MAML2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human MAML2 (internal sequence). The exact sequence is proprietary.
    Database link: Q8IZL2

  • Positive control

    • WB: HeLa whole cell and nuclear extract lysate. IP: HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 125 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.
  • Tissue specificity

    Widely expressed with high levels detected in placenta, salivary gland and skeletal muscle.
  • Involvement in disease

    Note=A chromosomal aberration involving MAML2 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with CRTC1/MECT1. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
  • Sequence similarities

    Belongs to the mastermind family.
  • Domain

    The C-terminal domain is required for transcriptional activation.
  • Cellular localization

    Nucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1819 antibody
    • Mam 2 antibody
    • Mam-2 antibody
    • MAML2 antibody
    • MAML2_HUMAN antibody
    • Mastermind-like protein 2 antibody
    see all

Images

  • All lanes : Anti-MAML2 antibody (ab226809) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa nuclear extract lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 125 kDa

  • MAML2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 5μg ab226809. Western blot was performed from the immunoprecipitate using ab226809.

    Lane 1: HeLa whole cell lysate (Input).
    Lane 2: Control IgG instead of ab226809 in HeLa whole cell lysate.
    Lane 3: ab226809 IP in HeLa whole cell lysate.

References

ab226809 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226809.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up