Key features and details
- Rabbit polyclonal to MAML2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAML2 antibody
See all MAML2 primary antibodies
DescriptionRabbit polyclonal to MAML2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment within Human MAML2 (internal sequence). The exact sequence is proprietary.
Database link: Q8IZL2
- WB: HeLa whole cell and nuclear extract lysate. IP: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 125 kDa.|
|IP||1/100 - 1/500.|
FunctionActs as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.
Tissue specificityWidely expressed with high levels detected in placenta, salivary gland and skeletal muscle.
Involvement in diseaseNote=A chromosomal aberration involving MAML2 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with CRTC1/MECT1. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
Sequence similaritiesBelongs to the mastermind family.
DomainThe C-terminal domain is required for transcriptional activation.
Cellular localizationNucleus speckle. Nuclear, in a punctate manner.
- Information by UniProt
- KIAA1819 antibody
- Mam 2 antibody
- Mam-2 antibody
All lanes : Anti-MAML2 antibody (ab226809) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa nuclear extract lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 125 kDa
MAML2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 5μg ab226809. Western blot was performed from the immunoprecipitate using ab226809.
Lane 1: HeLa whole cell lysate (Input).
Lane 2: Control IgG instead of ab226809 in HeLa whole cell lysate.
Lane 3: ab226809 IP in HeLa whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226809 has not yet been referenced specifically in any publications.