Key features and details
- Rabbit polyclonal to MAML2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAML2 antibody
See all MAML2 primary antibodies
DescriptionRabbit polyclonal to MAML2
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human MAML2 aa 250-300. The exact sequence is proprietary. AAK93831.1
Database link: Q8IZL2
- IP: MAML2 IP in HEK-293T whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245611 was affinity purified using an epitope specific to MAML2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.
Tissue specificityWidely expressed with high levels detected in placenta, salivary gland and skeletal muscle.
Involvement in diseaseNote=A chromosomal aberration involving MAML2 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with CRTC1/MECT1. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
Sequence similaritiesBelongs to the mastermind family.
DomainThe C-terminal domain is required for transcriptional activation.
Cellular localizationNucleus speckle. Nuclear, in a punctate manner.
- Information by UniProt
- KIAA1819 antibody
- Mam 2 antibody
- Mam-2 antibody
MAML2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245611 at 6 μg/reaction. Western blot was performed on the immunoprecipitate using an alternative anti-MAML2 antibody at 1 μg/ml.
Lane 1: ab245611 (Batch 1) IP in HEK-293T whole cell lysate.
Lane 2: ab245611 (Batch 2) IP in HEK-293T whole cell lysate.
Lane 3: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245611 has not yet been referenced specifically in any publications.