Overview

  • Product name

    Anti-MAML2 antibody [MAML2/1302]
    See all MAML2 primary antibodies
  • Description

    Mouse monoclonal [MAML2/1302] to MAML2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human MAML2 aa 1-1156.
    Database link: Q8IZL2

  • Positive control

    • Human pancreas, Human colon carcinoma, Human bladder carcinoma and Human placenta tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab218338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.
  • Tissue specificity

    Widely expressed with high levels detected in placenta, salivary gland and skeletal muscle.
  • Involvement in disease

    Note=A chromosomal aberration involving MAML2 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with CRTC1/MECT1. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
  • Sequence similarities

    Belongs to the mastermind family.
  • Domain

    The C-terminal domain is required for transcriptional activation.
  • Cellular localization

    Nucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1819 antibody
    • Mam 2 antibody
    • Mam-2 antibody
    • MAML2 antibody
    • MAML2_HUMAN antibody
    • Mastermind-like protein 2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human placenta tissue labeling MAML2 with ab218338 at 1 μg/ml dilution, followed by conjugation to the secondary antibody.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human bladder carcinoma tissue labeling MAML2 with ab218338 at 1 μg/ml dilution, followed by conjugation to the secondary antibody.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human colon carcinoma tissue labeling MAML2 with ab218338 at 1 μg/ml dilution, followed by conjugation to the secondary antibody.

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human pancreas tissue labeling MAML2 with ab218338 at 1 μg/ml dilution, followed by conjugation to the secondary antibody.

References

ab218338 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (MCF7(BREAST CANCER EPITHELIAL CELL))
Total protein in input
40 µg
Immuno-precipitation step
Protein A/G
Specification
MCF7(BREAST CANCER EPITHELIAL CELL)

Mr. sanoj naik

Verified customer

Submitted Sep 12 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (breast cancer)
Permeabilization
No
Specification
breast cancer
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 02 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (mammary epithelial (MCF7))
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Specification
mammary epithelial (MCF7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Aug 29 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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