Overview

  • Product name

    Anti-Mammaglobin A antibody [31A5]
    See all Mammaglobin A primary antibodies
  • Description

    Rabbit monoclonal [31A5] to Mammaglobin A
  • Host species

    Rabbit
  • Specificity

    Reacts with human mammaglobin A, no cross-reaction with lypophilin B and lypophilin C (mammaglobin B).
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Mammaglobin A.

  • Positive control

    • Breast carcinoma, skin (eccrine sweat glands).

Properties

Applications

Our Abpromise guarantee covers the use of ab82203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

Images

  • ab82203 at 1µg/ml staining Mammaglobin A in sentinel lymph nodes in metastasis of breast carcinoma.
  • ab82203 at 1µg/ml staining Mammaglobin A over-expression in breast carcinoma in situ.

References

This product has been referenced in:

  • Plante I  et al. Cx43 suppresses mammary tumor metastasis to the lung in a Cx43 mutant mouse model of human disease. Oncogene : (2010). IHC-P ; Mouse . Read more (PubMed: 21151177) »
  • Han JH  et al. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med 127:1330-4 (2003). WB, IHC-P, ELISA ; Human, Mouse, Monkey . Read more (PubMed: 14521461) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (4T1 breast tumor metastases in lung and liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton
Specification
4T1 breast tumor metastases in lung and liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 27 2015

Answer

Thank you for getting back to me and for confirming the order details. Currently, ab82203 is the only antibody against Mammaglobin A which has been tested for cross-reaction with mouse samples. Although, we have some other antibodies against the same target protein, but none of them have been used in murine tissues. I could certainly offer you a new vial of ab82203 as a free of charge replacement but it would be from the same batch (purification) since we do not have any new batch available yet. We are not aware of any batch-related quality problem so this may be a "one off" case and it may be worth trying a new vial if you wish. Please do let me know how you wish to proceed with your enquiry. I look forward to hearing from you soon.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up