Overview

  • Product name

    Anti-Mammaglobin A antibody [31A5]
    See all Mammaglobin A primary antibodies
  • Description

    Rabbit monoclonal [31A5] to Mammaglobin A
  • Host species

    Rabbit
  • Specificity

    Reacts with human mammaglobin A, no cross-reaction with lypophilin B and lypophilin C (mammaglobin B).
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Mammaglobin A.

  • Positive control

    • Breast carcinoma, skin (eccrine sweat glands).

Properties

Applications

Our Abpromise guarantee covers the use of ab82203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

Images

  • ab82203 at 1µg/ml staining Mammaglobin A in sentinel lymph nodes in metastasis of breast carcinoma.
  • ab82203 at 1µg/ml staining Mammaglobin A over-expression in breast carcinoma in situ.

References

This product has been referenced in:

  • Plante I  et al. Cx43 suppresses mammary tumor metastasis to the lung in a Cx43 mutant mouse model of human disease. Oncogene : (2010). IHC-P ; Mouse . Read more (PubMed: 21151177) »
  • Han JH  et al. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med 127:1330-4 (2003). WB, IHC-P, ELISA ; Human, Mouse, Monkey . Read more (PubMed: 14521461) »
See all 3 Publications for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (4T1 breast tumor metastases in lung and liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton
Specification
4T1 breast tumor metastases in lung and liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde

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Verified customer

Submitted May 27 2015

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