Key features and details
- Rabbit monoclonal [SP319] to Mammaglobin A - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Mammaglobin A antibody [SP319] - BSA and Azide free
See all Mammaglobin A primary antibodies
DescriptionRabbit monoclonal [SP319] to Mammaglobin A - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Mammaglobin A (C terminal). The exact sequence is proprietary.
Database link: Q13296
- IHC-P: Human breast ductal carcinoma tissue. Flow Cyt: MCF7 cells.
FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Ab240981 is the carrier-free version of ab223504. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240981 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab240981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
Primary antibody incubation for 30 minutes at 4 degrees.
|IHC-P||Use at an assay dependent concentration.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary Antibody Incubation: 10 minutes at room temperature.
Tissue specificityMammary gland specific. Over-expressed in breast cancer.
Sequence similaritiesBelongs to the secretoglobin family. Lipophilin subfamily.
- Information by UniProt
- Mammaglobin 1 antibody
- Mammaglobin-1 antibody
- Mammaglobin-A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Mammaglobin A with ab223504 at 1/100 dilution (1.08 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab223504 for 10 mins at room temperature.
This image was generated using ab223504, the same clone, but with a different buffer formulation.
Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells stained for Mammaglobin A using ab223504 (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab223504).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240981 has not yet been referenced specifically in any publications.