Key features and details
- Assay type: Direct
- Assay time: 20 min
- Sample type: Adherent cells, Suspension cells, Tissue
Product nameMammalian Cell Extraction Kit
Sample typeTissue, Adherent cells, Suspension cells
Assay time0h 20m
Abcam's Mammalian Cell Extraction Kit provides optimized cell extraction buffer, protease inhibitor cocktail, and DTT for convenient extraction of mammalian proteins from cultured cells and tissue samples, under nondenaturing conditions. Cell lysate prepared using the kit can be used in a variety of applications, such as enzyme activity assays (e.g. caspase activity assays), Western blot analysis, and others. The entire procedure takes less than 20 minutes.
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This product is manufactured by BioVision, an Abcam company and was previously called K269 Mammalian Cell Extraction Kit. K269-500 is the same size as the 500 test size of ab65399.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsPlease refer to protocols.
Components Identifier 500 tests Cell Extraction Buffer NM 1 x 100ml DTT 1 x 110µl Protease Inhibitor Cocktail Red 1 vial
RelevanceMammalian Cell Extraction methods allow extraction of mammalian proteins from cultured cells and tissue samples.
ab65399 has been referenced in 1 publication.
- Caglayan C et al. Rutin ameliorates mercuric chloride-induced hepatotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis. J Trace Elem Med Biol 56:60-68 (2019). PubMed: 31442956