Overview

  • Product name
    Mammalian Cell Lysis Buffer 5X
  • Sample type
    Adherent cells, Suspension cells
  • Product overview

    Mammalian Cell Lysis Buffer 5X (ab179835) is widely used to prepare mammalian cell and tissue lysates for use in a variety of downstream biochemical assays, especially those for quantification of enzymatic activity. This buffer does not contain SDS, a reagent that interferes with many activity assays.


    The lysis buffer has been optimized for mammalian samples, but it can also be used for preparation of plant or bacterial samples.


    The buffer just requires a simple 5-fold dilution, and minimal hands-on-time. Cell lysates are ready in only 15 minutes.

Properties

Protocols

References

This product has been referenced in:
  • Jehle J  et al. Genetic disruption of multidrug resistance-associated protein 1 improves endothelial function and attenuates atherosclerosis in MRP1-/- LDLr-/- double knockout mice. Arch Med Sci 13:930-936 (2017). Read more (PubMed: 28721160) »

See 1 Publication for this product

Customer reviews and Q&As

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The buffer is pH 7.


I can confirm that this lysis buffer does not contain SDS.

This lysis buffer can be used to lyse tissues and typically, we would recommend ˜400 ul for 20 mg of tissue.

Our Mammalian Cell Lysis Buffer 5X (ab179835) would be the best lysis buffer to use.

<1%Chaps also is OK. We have not checked NP40 and RIPA, but they should be OK theoretically.

The lysis buffer is an addition for the kit and it is not essential that you use this lysis buffer to prepare your samples. Most of our customers who are using 96 well plate based on 25 uL/well find that 10 ml of lysis buffer is sufficient.

If...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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