Product nameMammalian Cell Lysis Buffer 5X
Sample typeAdherent cells, Suspension cells
Mammalian Cell Lysis Buffer 5X (ab179835) is widely used to prepare mammalian cell and tissue lysates for use in a variety of downstream biochemical assays, especially those for quantification of enzymatic activity. This buffer does not contain SDS, a reagent that interferes with many activity assays.
The lysis buffer has been optimized for mammalian samples, but it can also be used for preparation of plant or bacterial samples.
The buffer just requires a simple 5-fold dilution, and minimal hands-on-time. Cell lysates are ready in only 15 minutes.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 ml Mammalian Cell lysis buffer 5X 1 x 10ml
This product has been referenced in:
- Ma X et al. Paxillin regulated genomic networks in prostate cancer. Steroids 151:108463 (2019). Read more (PubMed: 31344408) »
- Purnell MC et al. Modulation of endoplasmic reticulum stress and the unfolded protein response in cancerous and noncancerous cells. SAGE Open Med 6:2050312118783412 (2018). Read more (PubMed: 29977552) »