Overview

  • Product name

  • Description

    Rabbit polyclonal to MAN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to 18 amino acids near the C-terminus of Human MAN1 (NP_055134).

  • Positive control

    • Human colon tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab124148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.
  • Tissue specificity

    Heart, brain, placenta, lung, liver and skeletal muscle.
  • Involvement in disease

    Defects in LEMD3 are the cause of Buschke-Ollendorff syndrome (BOS) [MIM:166700]; also known as dermatoosteopoikilosis or disseminated dermatofibrosis with osteopoikilosis or dermatofibrosis lenticularis disseminata with osteopoikilosis or osteopathia condensans disseminata. BOS refers to the association of osteopoikilosis with disseminated connective-tissue nevi. Osteopoikilosis is a skeletal dysplasia characterized by a symmetric but unequal distribution of multiple hyperostotic areas in different parts of the skeleton. Both elastic-type nevi (juvenile elastoma) and collagen-type nevi (dermatofibrosis lenticularis disseminata) have been described in BOS. Skin or bony lesions can be absent in some family members, whereas other relatives may have both.
    Defects in LEMD3 are a cause of melorheostosis (MEL) [MIM:155950]. Melorheostosis is a rare mesenchymal dysplasia and one of the sclerosing bone disorders. It is caused by a developmental error, with a sclerotomal distribution, frequently involving one limb. It may be asymptomatic, but pain, stiffness with limitation of motion, leg-length discrepancy and limb deformity may occur.
  • Sequence similarities

    Contains 1 LEM domain.
  • Cellular localization

    Nucleus inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Inner nuclear membrane protein Man1 antibody
    • LEM domain containing protein 3 antibody
    • LEM domain-containing protein 3 antibody
    • LEMD3 antibody
    • MAN1_HUMAN antibody
    see all

Images

  • Anti-MAN1 antibody (ab124148) at 2 µg/ml + Human colon tissue lysate at 15 µg

    Predicted band size: 100 kDa

  • Immunohistochemical analysis of Human colon tissue, staining MAN1 with ab124148 at 2.5 µg/ml.

References

ab124148 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question

To whom it may concern:
As requested by a representative of Abcam, I send the information about the anti-Man1 antibodies that produce no staining.
Best wishes
=============================
1) Abcam product code: ab124148

2) Abcam order reference number or product batch number Lot No:
3) Description of the problem: did not stained nuclei periphery in cell and tissue samples

4) Sample preparation:
Species : mouse
Type of sample: fibroblasts in culture, cryosections
Sample preparation: freshly fixed cells, fixed mouse tissue
Positive control: -
Negative control: -

5) Fixation step: 4% formaldehyde in PBS
Fixation time: 10 min – for cells; 12h – for tissue
Fixation temperature: RT – for cells, 4ºC – for tissue

6) Antigen retrieval method:
Not performed for cells;
for cryosections: either no AR, or heating in sodium citrate buffer at +80ºC for 5, 10, 20, or 30 min

7) Permeabilization method:
0.5% Triton X100 in PBS, 10 min for cells, 1h for cryosections

8) Blocking agent: BSA
Concentration: 2%
Blocking time: 30 min
Blocking temperature: RT

9) Endogenous peroxidases blocked? No
Endogenous biotins blocked? No

10) Primary antibody:
Concentration or dilution 2.5 µg/ml
Diluent buffer: PBS
Incubation time: 1h – for cells; 12h – for sections

11) Secondary antibody:
Species: Donkey
Reacts against: Rabbit
Concentration or dilution: 1:500 – for cells; 1:250 – for sections
Diluent buffer: PBS
Incubation time: 1h – for cells; 12h – for sections
Fluorochrome or enzyme conjugate: Cy3

12) Washing after primary and secondary antibodies:
Buffer: PBS with 0.1% Tween – for cells; PBS with 0.1% TritonX100 – for sections
Number of washes: 3 x 10 min – for cells; 3 x 30 min – for sections

13) Detection method

14) How many times have you run this staining? x2 – cells; x2 - sections
Do you obtain the same results every time? yes
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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab124148 is tested and covered by our 6 month guarantee for use in mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab124148.

1.) MAN1 is a multi pass memebrane protein in the inner nuclear membrane. Triton it is not optimal for the use of membrane-associated antigens since it destroys membranes. I suggest to try a different permeabilisation agent like acetome or reduce the triton concentration to 0.1%.

2.) Could you confirm that the secondary antibody os working?

3.) In our experience this antibody has to be used less diluted when performing IF. I suggest 5ug/ml.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

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