Anti-Mannan Binding Lectin/MBL antibody [14D12] (ab106046)
Key features and details
- Rat monoclonal [14D12] to Mannan Binding Lectin/MBL
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
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Overview
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Product name
Anti-Mannan Binding Lectin/MBL antibody [14D12]
See all Mannan Binding Lectin/MBL primary antibodies -
Description
Rat monoclonal [14D12] to Mannan Binding Lectin/MBL -
Host species
Rat -
Specificity
The immunogen for this antibody is from Mouse MBL-2 (MBL-C).
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Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length native protein (purified) corresponding to Mouse Mannan Binding Lectin/MBL. The immunogen for this antibody is from Mouse MBL-2 (MBL-C).
Database link: P41317 -
Positive control
- IHC-P: Mouse Serum and kidney tissue
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General notes
It is recommended that solutions with a calcium concentration of 1 mM are used as ab106046 is a calcium-dependent antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution -
Clonality
Monoclonal -
Clone number
14D12 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/10.
Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4C in TBS/2% goat serum |
Notes |
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IHC-Fr
1/10. Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4C in TBS/2% goat serum |
Target
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Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 17195 Mouse
- SwissProt: P41317 Mouse
- Unigene: 30045 Mouse
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Alternative names
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
see all
Images
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ab106046 detecting Mannan Binding Lectin/MBL deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. Mannan Binding Lectin/MBL was detected in and around invading macrophages invading the intima (insert). Mannan Binding Lectin/MBL bound at sites of necrosis (upper right corner). No Mannan Binding Lectin/MBL binding was shown in the media or on fibrous caps covering the thickened intima.
Datasheets and documents
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Datasheet download
References (6)
ab106046 has been referenced in 6 publications.
- Bowie E & Goetz SC TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons. Elife 9:N/A (2020). PubMed: 31934864
- Garland DL et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). PubMed: 23943789
- Matthijsen RA et al. Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation 119:2188-95 (2009). PubMed: 19380618
- Held K et al. Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol 45:3934-41 (2008). PubMed: 18672286
- Phaneuf LR et al. Binding of mouse mannan-binding lectins to different bacterial pathogens of mice. Vet Immunol Immunopathol 118:129-33 (2007). PubMed: 17493687
- Liu H et al. Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scand J Immunol 53:489-97 (2001). PubMed: 11309157