Overview

  • Product name

    Anti-Mannan Binding Lectin/MBL antibody [14D12]
    See all Mannan Binding Lectin/MBL primary antibodies
  • Description

    Rat monoclonal [14D12] to Mannan Binding Lectin/MBL
  • Host species

    Rat
  • Specificity

    It is recommended that solutions with a calcium concentration of 1 mM are used as ab106046 is a calcium-dependent antibody.
  • Tested applications

    Suitable for: WB, ELISA, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length native protein (purified) corresponding to Mouse Mannan Binding Lectin/MBL.

  • Positive control

    • Mouse Serum and kidney tissue
  • General notes

     This product was previously labelled as Mannan Binding Lectin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    0.2 µm filtered antibody solution
  • Clonality

    Monoclonal
  • Clone number

    14D12
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10. Predicted molecular weight: 26 kDa. On a 12% non-reducing SDS-PAGE a prominent band of ~50Kda is detected. Under reducing conditions a 26 KDa band is detected.
ELISA Use at an assay dependent dilution. Biotinylated ab106046 was used as detector in ELISA at 1µg/ml TBS/Tween-20/Ca.
IHC-Fr 1/10. Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4C in TBS/2% goat serum
ICC/IF Use at an assay dependent dilution. Hyphae of C. albicans were fixed on poly-l-lysine coated slides with ice-cold acetone. Slides were incubated for 1h at RT with ab106046.

Target

  • Function

    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • Tissue specificity

    Plasma protein produced mainly in the liver.
  • Involvement in disease

    Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • COLEC 1 antibody
    • COLEC1 antibody
    • Collectin-1 antibody
    • HSMBPC antibody
    • Lectin, mannose-binding, soluble, 2 antibody
    • Mannan binding lectin antibody
    • Mannan binding protein antibody
    • Mannan-binding protein antibody
    • Mannose binding lectin (protein C) 2 soluble antibody
    • Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody
    • Mannose binding lectin (protein C) 2, soluble antibody
    • Mannose binding lectin 2 soluble antibody
    • Mannose binding lectin 2, soluble (opsonic defect) antibody
    • Mannose binding lectin antibody
    • Mannose binding lectin protein C2 soluble opsonic defect antibody
    • Mannose binding protein antibody
    • Mannose binding protein C antibody
    • Mannose binding protein C precursor antibody
    • Mannose binding protein, serum antibody
    • Mannose-binding lectin antibody
    • Mannose-binding protein C antibody
    • MBL 2 antibody
    • MBL antibody
    • MBL2 antibody
    • MBL2_HUMAN antibody
    • MBL2D antibody
    • MBP 1 antibody
    • MBP antibody
    • MBP C antibody
    • MBP-C antibody
    • MBP1 antibody
    • MBPB antibody
    • MBPC antibody
    • MBPD antibody
    • MGC116832 antibody
    • MGC116833 antibody
    • Opsonic defect antibody
    • protein C antibody
    • Soluble mannose binding lectin antibody
    see all

Images

  • ab106046 detecting Mannan Binding Lectin/MBL deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. Mannan Binding Lectin/MBL was detected in and around invading macrophages invading the intima (insert). Mannan Binding Lectin/MBL bound at sites of necrosis (upper right corner). No Mannan Binding Lectin/MBL binding was shown in the media or on fibrous caps covering the thickened intima.

References

This product has been referenced in:

  • Garland DL  et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23943789) »
  • Matthijsen RA  et al. Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation 119:2188-95 (2009). Read more (PubMed: 19380618) »
See all 5 Publications for this product

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