Recombinant
RabMAb

Recombinant Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834)

Overview

  • Product name

    Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156]
    See all Mannan Binding Lectin/MBL primary antibodies
  • Description

    Rabbit monoclonal [EPR18381-156] to Mannan Binding Lectin/MBL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein within Mouse Mannan Binding Lectin/MBL aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P41317

  • Positive control

    • WB: Mouse liver and kidney lysates; Mouse serum; Mouse Mannan Binding Lectin/MBL (aa19-244) recombinant protein. IHC-P: Mouse and human liver tissues.
  • General notes

     This product was previously labelled as Mannan Binding Lectin

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18381-156
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

This antibody is not suitable for human species in WB application due to non-specific staining and high background in human cell and tissue lysates.

IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • Tissue specificity

    Plasma protein produced mainly in the liver.
  • Involvement in disease

    Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • COLEC 1 antibody
    • COLEC1 antibody
    • Collectin-1 antibody
    • HSMBPC antibody
    • Lectin, mannose-binding, soluble, 2 antibody
    • Mannan binding lectin antibody
    • Mannan binding protein antibody
    • Mannan-binding protein antibody
    • Mannose binding lectin (protein C) 2 soluble antibody
    • Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody
    • Mannose binding lectin (protein C) 2, soluble antibody
    • Mannose binding lectin 2 soluble antibody
    • Mannose binding lectin 2, soluble (opsonic defect) antibody
    • Mannose binding lectin antibody
    • Mannose binding lectin protein C2 soluble opsonic defect antibody
    • Mannose binding protein antibody
    • Mannose binding protein C antibody
    • Mannose binding protein C precursor antibody
    • Mannose binding protein, serum antibody
    • Mannose-binding lectin antibody
    • Mannose-binding protein C antibody
    • MBL 2 antibody
    • MBL antibody
    • MBL2 antibody
    • MBL2_HUMAN antibody
    • MBL2D antibody
    • MBP 1 antibody
    • MBP antibody
    • MBP C antibody
    • MBP-C antibody
    • MBP1 antibody
    • MBPB antibody
    • MBPC antibody
    • MBPD antibody
    • MGC116832 antibody
    • MGC116833 antibody
    • Opsonic defect antibody
    • protein C antibody
    • Soluble mannose binding lectin antibody
    see all

Images

  • Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834) at 1/1000 dilution + Mouse Mannan Binding Lectin/MBL (aa19-244) recombinant protein at 0.005 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



     

    Exposure time : Lane 1: 3 seconds;  Lane 2: 3 minutes; Lane 3 1 second.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Mannan Binding Lectin/MBL with ab190834 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse liver is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Mannan Binding Lectin/MBL with ab190834 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab190834 has not yet been referenced specifically in any publications.

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