Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18381-83] to Mannan Binding Lectin/MBL
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse
Product nameAnti-Mannan Binding Lectin/MBL antibody [EPR18381-83]
See all Mannan Binding Lectin/MBL primary antibodies
DescriptionRabbit monoclonal [EPR18381-83] to Mannan Binding Lectin/MBL
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse
Recombinant full length protein within Mouse Mannan Binding Lectin/MBL aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P41317
- WB: Mouse serum; Mouse kidney lysate. IHC-P: Mouse liver tissue. IP: Mouse serum.
This product was previously labelled as Mannan Binding Lectin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab212060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
FunctionCalcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
Tissue specificityPlasma protein produced mainly in the liver.
Involvement in diseaseNote=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
Sequence similaritiesContains 1 C-type lectin domain.
Contains 1 collagen-like domain.
- Information by UniProt
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
All lanes : Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse kidney lysate
Lysates/proteins at 10 µg per lane.
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Line 1: 3 seconds: Lane 2: 41 seconds.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Mannan Binding Lectin/MBL with ab212060 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse liver (PMID: 12538708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Mannan Binding Lectin/MBL was immunoprecipitated from 0.35 mg of mouse serum with ab212060 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212060 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution
Lane 1: Mouse serum 10 µg (Input).
Lane 2: ab212060 IP in mouse serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212060 in mouse serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
ab212060 has not yet been referenced specifically in any publications.